Highly crosslinked vs conventional polyethylene particles - An in vitro comparison of biologic activities
Highly crosslinked vs conventional polyethylene particles - An in vitro comparison of biologic activities
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DOI：
10.1016/j.arth.2007.05.043
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发表时间：
2008-08-01
影响因子：
3.5
通讯作者：
Blanchard, Cheryl R.
中科院分区：
文献类型：
--
作者：
Illgen, Richard Lynn, II;Forsythe, Todd M.;Blanchard, Cheryl R.
高交联聚乙烯（hxpe）已被引入以减少髋关节置换术后的磨损。HXPE改善了磨损特性，但HXPE和常规聚乙烯（CPE）颗粒的相对生物活性尚不清楚。从髋关节模拟器中分离出Longevity （Zimmer, Warsaw, Ind； HXPE）和GUR 1050 （Zimmer； CPE）颗粒，并对其体外炎症反应（组织坏死因子a、白细胞介素1 α）进行了表征。和血管内皮生长因子水平)，使用巨噬细胞进行比较。长寿颗粒的平均直径（0.111 μ m）小于CPE颗粒（0.196 μ m），两者均以圆形（颗粒状）为主。HXPE和CPE的炎症反应呈浓度依赖性。低剂量（0.1表面积比[SAR]）和中剂量（0.75 SAR）没有统计学上的显著差异。在测试的最高剂量（2.5 SAR）下，基于相对组织坏死因子a和血管内皮生长因子分泌水平，HXPE的炎症性明显高于CPE。需要进一步的研究来确定类似的结果是否会在体内广泛的浓度范围内被注意到。
Highly cross-linked polyethylenes (HXPEs) have been introduced to reduce wear after hip arthroplasty. The improved wear characteristics of HXPEs are well documented, but the relative biologic activity of HXPE and conventional polyethylene (CPE) particles remains unclear. Longevity (Zimmer, Warsaw, Ind; HXPE) and GUR 1050 (Zimmer; CPE) particles were isolated and characterized from a hip simulator and their in vitro inflammatory responses (tissue necrosis factor a, interleukin 1 alpha., and vascular endothelial growth factor levels) were compared using macrophages. The average diameter of Longevity particles (0.111 mu m) was smaller than CPE particles (0.196 mu m), and both were predominantly round (granular appearance). The inflammatory response to HXPE and CPE was concentration dependent. No statistically significant differences were noted at low (0.1 surface area ratio [SAR]) and intermediate (0.75 SAR) doses. At the highest dose tested (2.5 SAR), HXPE was significantly more inflammatory than CPE based on relative tissue necrosis factor a and vascular endothelial growth factor secretion levels. Further study is needed to determine if similar findings would be noted in vivo over a broad concentration range.