Effect of a commercial probiotic on bacterial and phytoplankton concentration in intensive shrimp farming (Litopenaeus vannamei) recirculation systems
Effect of a commercial probiotic on bacterial and phytoplankton concentration in intensive shrimp farming (Litopenaeus vannamei) recirculation systems
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DOI：
10.3856/vol41-issue1-fulltext-10
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发表时间：
2013-03
影响因子：
1
通讯作者：
Enox de Paiva-Maia;George Alves-Modesto;Luis Otavio-Brito;A. Olivera;Tereza Cristina Vasconcelos-Gesteira
中科院分区：
文献类型：
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作者：
Enox de Paiva-Maia;George Alves-Modesto;Luis Otavio-Brito;A. Olivera;Tereza Cristina Vasconcelos-Gesteira
本研究的目的是评估一种商业益生菌对巴西北里奥格兰德河对虾精养循环系统中细菌和浮游植物浓度的影响。池塘平均面积为2.6公顷，养殖密度为98只虾m-2。一种商业益生菌是按照生产商的规格准备的，在放养前七天喷洒在池塘表面，然后每周喷洒一次，直到收获。对所有处理(对照和益生菌)使用相同的程序，包括喂养、浸灰、施肥、使用糖蜜和监测水质。使用方差分析、Tukey检验和卡方检验对现场数据进行分析。不同处理间的水质数据无显著差异，但处理方法对底泥中的总异养菌(5.181±0.34×10~4cfu g-1和5.749±0.67×10~4cfu g-1)、地表水中的总异养菌(4.514±0.95×10~4cfu m L~(-1)和4.136±0.81×10~4cfu m L~(-1))和地表水中的正蔗糖(2.438±0.72×10~4cfu m L~(-1)和2.203±0.76×10~4 cfu m L~(-1))有显著差异。用于对照和益生菌治疗。底泥中总异养菌、底泥中正、负蔗糖、表层水和底层水中总异养菌、甲藻百分含量在10周和16周之间也有显著差异。这些结果表明，益生菌能引起底泥中异养菌总数和甲藻浓度百分比值的变化，从而改善封闭循环系统池塘的底泥和水的环境质量。
The aim of this study was to evaluate the effect of a commercial probiotic on the bacterial and phytoplankton concentration in intensive shrimp farming (Litopenaeus vannamei) with a recirculation system, for one culture period in Rio Grande do Norte, Brazil. Ponds, with mean area of 2.6 ha, were stocked with a density of 98 shrimp m -2 . A commercial probiotic was prepared following the manufacture's specifications and sprayed on the surface of the ponds seven days prior to stocking and then on a weekly basis until harvest. The same procedures were used with all treatments (control and probiotic), with regard to feeding, liming, fertilization, use of molasses and monitoring of water quality. Field data were analyzed using ANOVA, the Tukey test and Chi-square tests. No significant differences between treatments were found for water quality data, but treatment means showed significant differences for total heterotrophic bacteria in the sediment (5.181 ± 0.34x10 4 cfu g -1 and 5.749 ± 0.67x10 4 cfu g -1 ), total heterotrophic bacteria in surface water (4.514 ± 0.95x 10 4 cfu m L -1 and 4.136 ± 0.81x10 4 cfu m L -1 ) and positive sucrose in surface water (2.438 ± 0.72x10 4 cfu m L -1 and 2.203 ± 0.76x10 4 cfu m L -1 ), respectively, for the control and probiotic treatment. Significant differences were also observed throughout the weeks for total heterotrophic bacteria in the sediment, positive and negative sucrose in the sediment, total heterotrophic bacteria in surface and bottom water, and Pyrrophyta percentage values between 10 and 16 weeks. These results showed that the probiotic causes changes in the total heterotrophic bacteria in the sediment and percentage values of Pyrrophyta concentration, improving the environmental quality of the sediment and water in ponds with closed recirculation systems.