Increase of Secondary Processes of Microglial and Astroglial Cells After MPTP-Induced Degeneration in Substantia Nigra Pars Compacta of Non Human Primates
Increase of Secondary Processes of Microglial and Astroglial Cells After MPTP-Induced Degeneration in Substantia Nigra Pars Compacta of Non Human Primates
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DOI：
10.1007/978-3-211-92660-4_20
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发表时间：
2009-01-01
影响因子：
--
通讯作者：
Herrero, Maria-Trinidad
中科院分区：
文献类型：
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作者：
Barcia, Carlos;Ros, Carmen M.;Herrero, Maria-Trinidad
帕金森病患者的黑质多巴胺能区显示出反应性星形胶质细胞和活性小胶质细胞的增加。这两种细胞类型的反应是与多巴胺能细胞损失相关的炎症反应的明确证据。然而，这种神经胶质反应的功能仍不清楚。这种组织学标志也在诱导的帕金森病动物如MPTP治疗的猴子中重现。在这项工作中，我们用共聚焦显微镜分析了MPTP处理的猴子SNPC中小胶质细胞和星形胶质细胞的突起数量，并与对照动物进行了比较。我们观察到，在MPTP处理的动物中，来自小胶质细胞和星形胶质细胞的次级分支增加，而初级分支的支架没有改变。这些结果表明，在MPTP治疗的猴子的胶质反应的特点是发射新的丝后，多巴胺能变性，这表明，胶质细胞可能会增加其扫描进程和修改后多巴胺能损伤的显微解剖。
Nigral dopaminergic areas from Parkinsonian patients show an increase of reactive astrocytes and active microglia. The reaction of these two cell types is a clear evidence of inflammatory response associated with dopaminergic cell loss. However, the function of this glial reaction remains unclear. This histological hallmark is also reproduced in induced Parkinsonian animals such as MPTP-treated monkeys. In this work, we analyze with confocal microscopy the number of processes of microglial cells and astrocytes in the SNpc of MPTP-treated monkeys and compare with control animals. We observe that secondary branches from microglia and astrocytes increase in MPTP-treated animals, while the scaffold of primary branches does not change. These results demonstrate that glial reaction in MPTP-treated monkeys is characterized by the emission of new filaments after the dopaminergic degeneration, suggesting that glial cells may increase their scanning progress and modify their microanatomy after dopaminergic injury.