A SIMPLE, NONCHROMATOGRAPHIC PROCEDURE TO PURIFY IMMUNOGLOBULINS FROM SERUM AND ASCITES FLUIDS
A SIMPLE, NONCHROMATOGRAPHIC PROCEDURE TO PURIFY IMMUNOGLOBULINS FROM SERUM AND ASCITES FLUIDS
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DOI：
10.1016/0022-1759(87)90324-3
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发表时间：
1987-02-11
影响因子：
2.2
通讯作者：
PARKINSON, A
中科院分区：
文献类型：
--
作者：
MCKINNEY, MM;PARKINSON, A
一个简单的，两个步骤的程序，以纯化免疫球蛋白G（IgG）级分从哺乳动物血清和腹水中描述。在第一步中，白蛋白和其他非IgG蛋白质用辛酸（辛酸）沉淀。在第二种方法中，用硫酸铵沉淀IgG级分。影响辛酸沉淀血清蛋白的因素进行了描述，是程序的修改，以纯化从血清中的IgG馏分具有高的脂质含量。该方法可用于从兔、绵羊、山羊、马、大鼠和小鼠的血清中纯化IgG组分，以及从小鼠腹水中纯化单克隆抗体。用此方法可从兔血清中分离出80%以上的IgG，其纯度与阴离子交换层析法纯化的IgG相当。除了其简单性和低成本之外，所描述的方法提供了优于其他方法的几个优点来纯化IgG。
A simple, two-step procedure to purify the immunoglobulin G (IgG) fraction from mammalian sera and ascites fluid is described. In the first step, albumin and other non-IgG proteins are precipitated with caprylic acid (octanoic acid). In the second, the IgG fraction is precipitated with ammonium sulfate. Factors influencing the precipitation of serum proteins by caprylic acid are described, as are procedural modifications to purify the IgG fraction from sera with a high lipid content. The procedure can be used to purify the IgG fraction of serum from rabbit, sheep, goat, horse, rat and mouse, as well as monoclonal antibodies from mouse ascites fluid. Greater than 80% of the IgG in rabbit serum could be isolated by this procedure, with a purity equal to rabbit IgG purified by anion-exhange chromatography. In addition to its simplicity and low cost, the procedure described offers several advantages over other methods to purify IgG.