DNA-Based Photoacoustic Nanosensor for Interferon Gamma Detection.
DNA-Based Photoacoustic Nanosensor for Interferon Gamma Detection.
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DOI：
10.1021/acssensors.9b00209
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发表时间：
2019-04
期刊：
影响因子：
8.9
通讯作者：
Jennifer M. Morales;R. Pawle;N. Akkilic;Yi Luo;Marvin Xavierselvan;Rayan Albokhari;I. Calderon;Scott Selfridge;R. Minns;L. Takiff;S. Mallidi;H. Clark
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文献类型：
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作者：
Jennifer M. Morales;R. Pawle;N. Akkilic;Yi Luo;Marvin Xavierselvan;Rayan Albokhari;I. Calderon;Scott Selfridge;R. Minns;L. Takiff;S. Mallidi;H. Clark
跟踪体内的蛋白质水平在研究和医学中都是至关重要的，了解它们的生理作用可以深入了解它们在体内平衡和疾病中的调节。在医学中，蛋白质水平是主动采样的，因为它们不断波动，反映了生物系统的状态，并提供了对患者健康的洞察。一种这样的蛋白质是干扰素γ，一种具有免疫调节功能的临床相关蛋白质，其在抗感染中起关键作用。用于连续监测体内蛋白质水平的新工具在监测患者的实时状况以提供更好的护理方面是非常宝贵的。在这里，我们开发了一种基于DNA的纳米传感器，用于干扰素γ的光声检测。这项工作展示了我们如何将一个简单的DNA基序，受体和一种新型的酞菁染料转化为用于蛋白质检测的概念验证光声纳米传感器。表面等离子体共振动力学分析表明，纳米传感器是响应和可逆的干扰素γ的亲和力在纳摩尔范围内，KD 1 = 167 nM和KD 2 = 316 nM。作为报告者，我们的设计包括一种新型的基于酞菁的光声染料，该染料以J聚集体堆叠，导致信号增加22.5%。在受体结合时，DNA结构弯曲以诱导酞菁染料堆积，导致在10 μM干扰素γ存在下光声信号增加55%。这种概念验证纳米传感器是开发光声传感器的一种新方法，并且可能适用于未来的其他感兴趣的蛋白质，用于体内跟踪。
Tracking protein levels in the body is vital in both research and medicine, where understanding their physiological roles provides insight into their regulation in homeostasis and diseases. In medicine, protein levels are actively sampled since they continuously fluctuate, reflecting the status of biological systems and provide insight into patient health. One such protein is interferon gamma, a clinically relevant protein with immunoregulatory functions that play critical roles against infection. New tools for continuously monitoring protein levels in vivo are invaluable in monitoring real-time conditions of patients to allow better care. Here, we developed a DNA-based nanosensor for the photoacoustic detection of interferon gamma. This work demonstrates how we transformed a simple DNA motif, receptors, and a novel phthalocyanine dye into a proof-of-concept photoacoustic nanosensor for protein detection. Surface plasmon resonance kinetic analysis demonstrated that the nanosensor is responsive and reversible to interferon gamma with an affinity in the nanomolar range, KD1 = 167 nM and KD2 = 316 nM. As a reporter, our design includes a novel phthalocyanine-based photoacoustic dye that stacks in a J-aggregate, causing a 22.5% increase in signal. Upon receptor binding, the DNA structure bends to induce phthalocyanine dye stacking, resulting in a 55% increase in photoacoustic signal in the presence of 10 μM interferon gamma. This proof-of-concept nanosensor is a novel approach to the development of a photoacoustic sensor and may be adapted for other proteins of interest in the future for in vivo tracking.