Salidroside promotes erythropoiesis and protects erythroblasts against oxidative stress by up-regulating glutathione peroxidase and thioredoxin
Salidroside promotes erythropoiesis and protects erythroblasts against oxidative stress by up-regulating glutathione peroxidase and thioredoxin
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DOI：
10.1016/j.jep.2010.09.025
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发表时间：
2011-01-27
影响因子：
5.4
通讯作者：
Kong, Siu-Kai
中科院分区：
文献类型：
--
作者：
Qian, Erin Wei;Ge, Daniel Tianfang;Kong, Siu-Kai
民族药理学相关性：红景天是中国和西藏民间常用的药物，用于治疗高原反应、缺氧和山地缺氧。研究目的：红景天苷(SDS)是红景天的有效成分。本研究试图探讨 SDS 在 TF-1 成红细胞中潜在的红细胞生成刺激和抗氧化作用。 材料和方法：通过测量一系列红细胞标记物的表达，在存在和不存在促红细胞生成素 (EPO) 的情况下，用 SDS 处理人 TF-1 细胞（研究红细胞生成的流行体外模型之一）来确定促红细胞生成作用。如血型糖蛋白 A (GPA)、转铁蛋白受体 (CD71) 和血红蛋白 (Hb)。通过流式细胞术和蛋白质印迹分析检测SDS对H2O2诱导的TF-1成红细胞凋亡的潜在保护作用及其潜在机制。结果：SDS促进EPO处理的细胞中的红细胞生成，并且还减少H2O2处理后TF-1成红细胞中凋亡细胞的数量，可能是通过上调保护蛋白硫氧还蛋白-1（Trx1）和谷胱甘肽来实现的。过氧化物酶-1 (GPx1)。结论：我们的研究为解释 SDS 和红景天在中药中的民族药理学作用提供了证据。我们的研究结果还支持使用 SDS 作为红细胞生成辅助剂来纠正贫血和缺氧。 (C) 2010 Elsevier Ireland Ltd. 保留所有权利。
Ethnopharmacological relevance: Rhodiola rosea is commonly used in China and Tibet folk medicine for the treatment of high altitude sickness, anoxia and mountain malhypoxia.Aim of study: Salidroside (SDS) is an active ingredient of Rhodiola rosea. This study attempted to examine the potential erythropoiesis-stimulating and anti-oxidative effect of SDS in TF-1 erythroblasts.Materials and methods: The erythropoiesis-promoting effect was determined by treating human TF-1 cells, one of the popular in vitro models for studying erythropoiesis, with SDS in the presence and absence of erythropoietin (EPO) through the measurement of the expression of a series of erythroid markers such as glycophorin A (GPA), transferrin receptor (CD71) and hemoglobin (Hb). The potential protective effect of SDS against H2O2-induced apoptosis and its underlying mechanism in TF-1 erythroblasts were examined by flow cytometry and Western blot analysis.Results: SDS promotes erythropoiesis in the EPO-treated cells and it also reduces the number of apoptotic cells in TF-1 erythroblasts after H2O2 treatment probably through the up-regulation of protective proteins thioredoxin-1 (Trx1) and glutathione peroxidase-1 (GPx1).Conclusion: Our study provides evidence to explain the ethnopharmacological role of SDS and Rhodiola rosea in Chinese medicine. Our findings also support the use of SDS as an erythropoiesis-adjuvant agent to correct anemia and malhypoxia. (C) 2010 Elsevier Ireland Ltd. All rights reserved.