Time-lapse epifluorescence imaging of expressed cRNA to cyclin B1 for studying meiosis I in mouse oocytes.
Time-lapse epifluorescence imaging of expressed cRNA to cyclin B1 for studying meiosis I in mouse oocytes.
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DOI：
10.1007/978-1-62703-191-2_6
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发表时间：
2013-01-01
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影响因子：
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通讯作者：
Jones, Keith T
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文献类型：
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作者：
Holt, Janet E;Lane, Simon I R;Jones, Keith T
哺乳动物卵母细胞的第一次减数分裂在生理上发生在排卵前的几个小时。幸运的是，从卵泡环境中取出的卵母细胞在培养中很容易经历这一过程。它们的大尺寸，光学透明度和翻译外源cRNA的效率使得小鼠卵母细胞非常适合使用基于荧光成像的技术详细研究这一过程。在这里，我们描述的过程中显微注射cRNA的目的蛋白质，已被耦合到一个荧光蛋白使用细胞周期蛋白B1作为一个例子。
The first meiotic division of mammalian oocytes physiologically occurs in the ovary in the hours preceding ovulation. Fortunately, oocytes removed from their follicular environment will readily undergo this process in culture. Their large size, optical transparency, and efficiency in translating exogenous cRNA make mouse oocytes very amenable to study this process in detail using fluorescence imaging-based techniques. Here we describe the process of microinjecting cRNA to proteins of interest that have been coupled to a fluorescent protein using cyclin B1 as an example.