Probing Cell Deformability via Acoustically Actuated Bubbles.
Probing Cell Deformability via Acoustically Actuated Bubbles.
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DOI：
10.1002/smll.201502220
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发表时间：
2016-02-17
期刊：
影响因子：
--
通讯作者：
Huang TJ
中科院分区：
文献类型：
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作者：
Xie Y;Nama N;Li P;Mao Z;Huang PH;Zhao C;Costanzo F;Huang TJ
开发了一种基于声致动的气泡技术来研究悬浮在微流控装置中的细胞的变形性。通过在悬浮在微流体室中的靶细胞附近的光热效应产生微米尺寸的气泡。随后，采用声致动来产生局部声流。反过来，流动导致流体动力，使细胞原位变形。细胞的可变形性是其机械性质的指示。本研究中的方法在一次实验中测量多个细胞的机械生物标志物，并且可以方便地与其他生物分析和药物筛选平台集成。使用该技术，测量数十个HeLa、HEK和HUVEC细胞的平均变形性以区分它们的机械性质。HeLa细胞在用细胞松弛素处理后变形。该技术还通过使用荧光标记物和机械生物标记物揭示了混合的异质细胞样品中每个亚群的变形性。这项研究中的技术，除了与细胞生物学相关外，还将使生物物理细胞诊断成为可能。
An acoustically actuated, bubble-based technique is developed to investigate the deformability of cells suspended in microfluidic devices. A microsized bubble is generated by an optothermal effect near the targeted cells, which are suspended in a microfluidic chamber. Subsequently, acoustic actuation is employed to create localized acoustic streaming. In turn, the streaming flow results in hydrodynamic forces that deform the cells in situ. The deformability of the cells is indicative of their mechanical properties. The method in this study measures mechanical biomarkers from multiple cells in a single experiment, and it can be conveniently integrated with other bioanalysis and drug-screening platforms. Using this technique, the mean deformability of tens of HeLa, HEK, and HUVEC cells is measured to distinguish their mechanical properties. HeLa cells are deformed upon treatment with Cytochalasin. The technique also reveals the deformability of each subpopulation in a mixed, heterogeneous cell sample by the use of both fluorescent markers and mechanical biomarkers. The technique in this study, apart from being relevant to cell biology, will also enable biophysical cellular diagnosis.