The protein kinase kin1, the fission yeast orthologue of mammalian MARK/PAR-1, localises to new cell ends after mitosis and is important for bipolar growth
The protein kinase kin1, the fission yeast orthologue of mammalian MARK/PAR-1, localises to new cell ends after mitosis and is important for bipolar growth
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DOI：
10.1016/s0014-5793(03)01080-9
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发表时间：
2003-11-06
期刊：
影响因子：
3.5
通讯作者：
Nurse, P
中科院分区：
文献类型：
--
作者：
Drewes, G;Nurse, P
裂殖酵母的K1蛋白激酶是PAR-1/Mark(Partiding-Defect 1/Microtubule-Association Protein/Microtubule Affiliation-Reguling Kinase)家族的成员，在真核细胞的极性和细胞骨架动力学中起重要作用。我们在这里展示了Kin1在建立分裂酵母特有的杆状形态中所起的作用。Kin1缺失的细胞是有活力的，但生长受阻，一端或两端是圆形的。它们是单极的，因为有丝分裂后它们不能激活双极生长，胞质分裂延迟，导致高比例的分隔细胞通常具有多个隔膜。人类标记的异源表达可以部分挽救这种表型，这可以恢复大多数细胞的两极生长，但不能纠正细胞质分裂的延迟。利用染色体表位标记，我们发现Kin1p定位于细胞末端，除了在有丝分裂期间它从细胞末端消失之外。有丝分裂后，kin1p首先出现在新的细胞端。Kin1的过度表达导致极性的丧失，细胞部分或完全圆形。根据这些结果，我们认为Kin1是将生长机械引导到细胞末端所必需的。(C)2003年，由Elsevier B.V.代表欧洲生化学会联合会出版。
The kin1 protein kinase of the fission yeast Schizosaccharomyces pombe is a member of the PAR-1/MARK (partitioning-defective 1/microtubule-associated protein/microtubule affinity-regulating kinase) family important in eukaryotic cell polarity and cytoskeletal dynamics. We show here that kin1 plays a role in establishing the characteristic rod-shaped morphology of fission yeast. Cells in which kin1 was deleted are viable but are impaired in growth, and are rounded at one end or both ends. They are monopolar because after mitosis they fail to activate bipolar growth, and are delayed in cytokinesis, resulting in a high proportion of septated cells often with multiple septa. This phenotype can be partially rescued by heterologous expression of human MARKs, which restore bipolar growth in most cells, but do not correct the delay in cytokinesis. Using chromosomal epitope tagging, we show that kin1p localises to the cell ends, except during mitosis when it disappears from cell ends. After mitosis, kin1p first reappears at the new cell end. Overexpression of kin1 results in a loss of polarity, with partially or fully rounded cells. From these results we suggest that kin1 is required to direct the growth machinery to the cell ends. (C) 2003 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.