Epigenetic reprogramming of nuclei using cell extracts
Epigenetic reprogramming of nuclei using cell extracts
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DOI：
10.1007/bf02698058
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发表时间：
2006-01-01
期刊：
影响因子：
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通讯作者：
Taranger, Christel K.
中科院分区：
文献类型：
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作者：
Collas, Philippe;Taranger, Christel K.
最近的证据表明，未分化细胞的核和细胞质提取物可以重新编程基因表达，并促进其他发育受限的细胞类型的多能性。值得注意的是，胚胎癌细胞或胚胎干细胞的提取物已被证明可以引起靶细胞转录程序的转变，从而上调胚胎干细胞基因，下调体细胞特异性标记物，并对组蛋白进行表观遗传修饰。重编程的肾上皮细胞获得向外胚层和中胚层谱系分化的潜力。细胞提取物介导的核重编程可能是通过细胞融合或核移植重编程体细胞的一种有吸引力的替代方案。这篇综述强调了最近的观察结果，得出了这样一个概念：多能细胞提取物含有能够重新编程体细胞核功能的调节成分。还解决了当前基于提取的重编程方法的局限性。
Recent evidence indicates that nuclear and cytoplasmic extracts from undifferentiated cells can reprogram gene expression and promote pluripotency in otherwise more developmentally restricted cell types. Notably, extracts of embryonal carcinoma cells or embryonic stem cells have been shown to elicit a shift in the transcriptional program of target cells to upregulate embryonic stem cell genes, downregulate somatic cell-specific markers, and epigenetically modify histones. Reprogrammed kidney epithelial cells acquire a potential for differentiation toward ectodermal and mesodermal lineages. Cell extract-mediated nuclear reprogramming may constitute an attractive alternative to reprogramming somatic cells by cell fusion or nuclear transfer. This review highlights recent observations leading to the concept that extracts derived from pluripotent cells contain regulatory components capable of reprogramming somatic nuclear function. Limitations of current extract-based reprogramming approaches are also addressed.