COLD1 Confers Chilling Tolerance in Rice
COLD1 Confers Chilling Tolerance in Rice
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DOI：
10.1016/j.cell.2015.01.046
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发表时间：
2015-03-12
期刊：
影响因子：
64.5
通讯作者：
Chong, Kang
中科院分区：
文献类型：
--
作者：
Ma, Yun;Dai, Xiaoyan;Chong, Kang
水稻对寒冷很敏感，只能在某些气候区种植。人类对粳稻的选择将其生长区域扩展到温度较低的地区，而这种适应的分子基础尚不清楚。在这里，我们确定了赋予粳稻耐冷性的数量性状位点COLD1。COLD1（jap）过表达显著增强了水稻的冷耐受性，而COLD1（jap）缺失或下调的水稻品系对冷敏感。COLD1编码一个定位于质膜和内质网（ER）的g蛋白信号调节因子。它与g蛋白α亚基相互作用，激活Ca2+通道以感知低温，并加速g蛋白GTPase活性。我们进一步确定了COLD1中的一个SNP SNP2，来自中国Oryza rufipogon，负责coldap /ind赋予低温耐受性的能力，支持COLD1在植物适应中的重要性。
Rice is sensitive to cold and can be grown only in certain climate zones. Human selection of japonica rice has extended its growth zone to regions with lower temperature, while the molecular basis of this adaptation remains unknown. Here, we identify the quantitative trait locus COLD1 that confers chilling tolerance in japonica rice. Overexpression of COLD1(jap) significantly enhances chilling tolerance, whereas rice lines with deficiency or downregulation of COLD1(jap) are sensitive to cold. COLD1 encodes a regulator of G-protein signaling that localizes on plasma membrane and endoplasmic reticulum (ER). It interacts with the G-protein alpha subunit to activate the Ca2+ channel for sensing low temperature and to accelerate G-protein GTPase activity. We further identify that a SNP in COLD1, SNP2, originated from Chinese Oryza rufipogon, is responsible for the ability of COLDjap/ind to confer chilling tolerance, supporting the importance of COLD1 in plant adaptation.