Candidate genes and QTLs for fruit ripening and softening in melon
Candidate genes and QTLs for fruit ripening and softening in melon
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DOI：
10.1007/s00122-007-0694-y
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发表时间：
2008-02-01
影响因子：
5.4
通讯作者：
Garcia-Mas, Jordi
中科院分区：
文献类型：
--
作者：
Moreno, Eduard;Obando, Javier M.;Garcia-Mas, Jordi
不同的因素影响瓜类水果的质量，从消费者的角度来看，其中长的保质期是至关重要的。在甜瓜中，发现了表现出跃变型和非跃变型成熟类型的品种，在本研究中，我们使用来自非跃变型甜瓜亲本系PI 161375（SC）和“Piel de Sapo”（PS）的近等基因系（NILs）的集合来研究跃变型成熟和果实软化。令人惊讶的是，我们发现QTL eth3.5在NIL SC 3 - 5 b诱导呼吸和乙烯水平增加的更年期成熟表型。数据表明，来自PI 161375和“Piel de Sapo”的非更年期表型可能是不同基因突变的结果。同时检测到多个影响果肉硬度的QTL。定位了参与乙烯调控、乙烯生物合成与感知、细胞壁降解等功能的候选基因pupp，并观察到与QTL的共定位。这些结果可能为理解甜瓜的非跃变型成熟提供额外的数据。
Different factors affect the quality of melon fruit and among them long shelf life is critical from the consumer's point of view. In melon, cultivars showing both climacteric and non-climacteric ripening types are found. In this study we have investigated climacteric ripening and fruit softening using a collection of near-isogenic lines (NILs) derived from the non-climacteric melon parental lines PI 161375 (SC) and "Piel de Sapo" (PS). Surprisingly, we found that QTL eth3.5 in NIL SC3-5b induced a climacteric-ripening phenotype with increased respiration and ethylene levels. Data suggest that the non-climacteric phenotypes from PI 161375 and "Piel de Sapo" may be the result of mutations in different genes. Several QTLs for fruit flesh firmness were also detected. Candidate genes putatively involved in ethylene regulation, biosynthesis and perception and cell wall degradation were mapped and some colocations with QTLs were observed. These results may provide additional data towards understanding of non-climacteric ripening in melon.