Microvesicles in bronchoalveolar lavage as a potential biomarker of COPD
Microvesicles in bronchoalveolar lavage as a potential biomarker of COPD
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DOI：
10.1152/ajplung.00362.2020
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发表时间：
2021-02-01
影响因子：
4.9
通讯作者：
Saetta, Marina
中科院分区：
文献类型：
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作者：
Bazzan, Erica;Radu, Claudia Maria;Saetta, Marina
几乎所有细胞释放的微泡（MV）被认为是细胞通讯工具。 MV 已在多种炎症性疾病中进行了研究，但在生物体液（例如吸烟者的支气管肺泡灌洗液 (BAL)）中的研究却很少。本研究的目的是调查患有或不患有慢性阻塞性肺疾病 (COPD) 的吸烟者与不吸烟对照者 BAL 中 MV 的存在和来源。使用 BAL 中的流式细胞术，我们检测了内皮和肺泡巨噬细胞 (AM) 衍生的 MV，发现患有 COPD 的吸烟者的 BAL 中 AM-MV 的数量高于无 COPD 的吸烟者和非吸烟者，这与包年数相关 (r = 0.46；P = 0.05) 以及通过预测的 1 秒用力呼气量测量的气道阻塞程度 (r = 0.56；P = 0.01）。人 BAL 液中存在并可测量内皮和肺泡巨噬细胞衍生的 MV。为了响应吸烟和 COPD 的发展，AM 衍生的 MV 中的炎症信号可以被量化，它们的数量与包年数和肺功能下降有关。这些结果为未来研究这些微泡作为慢性阻塞性肺病的生物标志物和可能的机制指导提供了机会。
Microvesicles (MVs) released from almost all cells are recognized as cell communication tools. MVs have been investigated in several inflammatory diseases but poorly in biological fluids like bronchoalveolar lavage (BAL) of smokers. The purpose of this study was to investigate the presence and source of MVs in BAL of smokers with and without chronic obstructive pulmonary disease (COPD) compared with nonsmoking controls. Using flow cytometry in BAL, we detected endothelial and alveolar macrophage (AM)-derived MVs and found a higher number of AM-MVs in the BAL of smokers with COPD than in smokers without COPD and nonsmokers, which correlated with the pack-years (r = 0.46; P = 0.05) and with the degree of airway obstruction measured by the forced expiratory volume in 1 s percent predicted (r = 0.56; P = 0.01). Endothelial and alveolar macrophage-derived MVs are present and measurable in human BAL fluid. In response to smoking and to the development of COPD, inflammatory signals in AM-derived MVs can be quantified, and their numbers are related to the pack-years and the decrease in lung function. These results open the opportunity for future investigation of these microvesicles as biomarkers and possible mechanistic guides in COPD.