Investigation of antibiotic resistance profile and TEM-type β-lactamase gene carriage of ampicillin-resistant Escherichia coli strains isolated from drinking water
Investigation of antibiotic resistance profile and TEM-type β-lactamase gene carriage of ampicillin-resistant Escherichia coli strains isolated from drinking water
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DOI：
10.1007/bf03175221
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发表时间：
2007-01-01
影响因子：
3
通讯作者：
Yesilgil, Pinar
中科院分区：
文献类型：
--
作者：
Alpay-Karaoglu, Sengul;Ozgumus, Osman Birol;Yesilgil, Pinar
2000 年至 2002 年以及 2004 年 1 月至 2004 年 2 月，从土耳其 Rize 地区淡水资源丰富的 51 个饮用水点中分离出 55 株耐氨苄青霉素（Amp(r)）大肠杆菌菌株。大量微生物（近 57%）对人类和兽医常用的三种或多种抗生素表现出耐药性，这些菌株表现出多重耐药性。表型。近一半的菌株（27%）对头孢他啶表现出耐药性，但根据双盘协同试验结果，这些菌株并不是超广谱β-内酰胺酶产生者。然后通过聚合酶链反应筛选所有分离株是否携带 TEM 型 β-内酰胺酶基因 (bla(TEM))。在 6 个（11%）分离株中发现了 TEM 型 P-内酰胺酶基因。序列分析显示TEM-1型基因。然而，除了一株菌株外，等电聚焦分析并未证实TEM-1型β-内酰胺酶的产生。接合实验表明，对氨苄西林、四环素或甲氧苄啶/磺胺甲恶唑的耐药性在六株（11%）分离株中是可转移的。公共饮用水中大肠杆菌菌株中出现的可转移抗生素耐药性和 bla(TEM-1) 基因对公共健康构成重大风险。
Fifty-five ampicillin-resistant (Amp(r)) Escherichia coli strains were isolated from 51 drinking water points in Rize region containing abundant fresh water sources in Turkey during the years 2000 to 2002 and from January to February 2004. The large number of organisms (nearly 57%) exhibited resistance to three or more antibiotics commonly used in human and veterinary medicine, These strains displayed a multiresistant phenotype. Nearly half of the strains (27%) expressed resistance to ceftazidime, but these strains were not an extended-spectrum beta-lactamase-producer according to the results of double-disk synergy test. All isolates were then screened for the carriage of TEM-type beta-lactamase gene (bla(TEM)) by polymerase chain reaction. TEM-type P-lactamase genes were found in six (11%) isolates. Sequence analysis showed TEM-1 type genes. However, isoelectric focusing analysis did not confirm the production of TEM-1 type P-lactamase except for one strain, Conjugation experiments showed that resistance to ampicillin, tetracycline or trimethoprim/sulfamethoxazole was transferable in six (11%) isolates. Emergence of transferable antibiotic resistance and bla(TEM-1) gene in E. coli strains from public drinking waters possesses a significant public health risk.