Mycorrhizal synthesis of Tuber indicum with two indigenous hosts, Castanea mollissima and Pinus armandii
Mycorrhizal synthesis of Tuber indicum with two indigenous hosts, Castanea mollissima and Pinus armandii
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DOI：
10.1007/s00572-009-0247-0
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发表时间：
2009-09-01
期刊：
影响因子：
3.9
通讯作者：
Shen, Yu-Ying
中科院分区：
文献类型：
--
作者：
Geng, Li-Ying;Wang, Xiang-Hua;Shen, Yu-Ying
印度块菌（Tuber indicum）是我国最著名的商业真菌之一。然而，菌根调查主要是用外来树种进行的。迄今为止，还没有详细的形态学描述与T。印度以华山松和板栗两种中国西南地区特有寄主的容器苗为材料，接种T. indicum在两种基质中的生长情况。接种后4个月开始形成真菌，9个月时收获。确定该真菌为T.通过形态学和ITS-rDNA序列分析，对印度棉进行了初步的鉴定。发散菌丝和表皮样外套膜外观的形态与一些欧洲树木获得的真菌相似。由于其高度的形态变异和与黑孢块菌的相似性，很难仅从形态上区分这两种真菌。T. indicum与其本土宿主的关系将对亚洲黑松露的计划种植具有重要意义。
Tuber indicum is one of the most renowned commercialized fungi in China. Mycorrhizal investigations, however, have been carried out mainly with exotic trees. Up to now there is no detailed description of morphology of the mycorrhizae formed with the indigenous hosts of T. indicum. Containerized seedlings of two indigenous hosts of the fungus in southwestern China, Pinus armandii and Castanea mollissima, were inoculated with aqueous spore suspension of T. indicum in two kinds of substrates. Mycorrhizae began to form 4 months after inoculation and were harvested at 9 months. The contributing fungus of the mycorrhizae was confirmed to be T. indicum by morphological and ITS-rDNA sequence analyses. The morphology of emanating hyphae and epidermoid-like mantle appearance was similar to the mycorrhizae obtained with some European trees. The high morphological variation and the similarity to that of Tuber melanosporum makes it difficult to distinguish the mycorrhizae of the two species by morphology alone. The synthesis of mycorrhizae of T. indicum with its indigenous hosts will be of great significance for planned cultivation of the Asian black truffles.