The curved DNA structure in the 5′-upstream region of the light-responsive genes:: its universality, binding factor and function for cyanobacterial psbA transcription
The curved DNA structure in the 5′-upstream region of the light-responsive genes:: its universality, binding factor and function for cyanobacterial psbA transcription
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DOI：
10.1093/nar/gkf605
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发表时间：
2002-11-01
影响因子：
14.9
通讯作者：
Ohyama, T
中科院分区：
文献类型：
--
作者：
Asayama, M;Kato, H;Ohyama, T
在单细胞光合蓝细菌铜绿微囊藻K-81中，在psbA 2基因的5 '上游区域发现了独特的DNA弯曲CIT，该基因表现出基础、光响应和昼夜节律的转录。在这项研究中，我们报告了在psbA家族的5 '-上游区域中发现的曲率的普遍性以及曲率在基因表达中的功能。在另一种蓝细菌，一种红色的蓝藻和植物（单子叶植物和双子叶植物）的psbA基因上游1000 bp内确定了内在曲率。构建了诱变的弯曲，并通过凝胶电泳和原子力显微镜证实其具有破坏的结构。在光/暗和光/强光条件下，在含有诱变曲率的蓝藻转化体中观察到相对少量但光响应的psbA 2转录物。这表明曲率对于基础转录是重要的。体外引物延伸和DNA迁移率变动分析表明，可能与弯曲中心上游区域结合的因子有助于psbA 2的有效基础转录。
A unique DNA curvature, the CIT, has been found in the 5'-upstream region of the psbA2 gene, which exhibits basal, light-responsive and circadian rhythmic transcription, in a unicellular photosynthetic cyanobacterium, Microcystis aeruginosa K-81. In this study, we report the universality of curvatures found in 5'-upstream regions in the psbA family and the function of the curvature in gene expression. Intrinsic curvatures were identified within 1000 bp upstream from the psbA genes in another cyanobacterium, a red alga and in plants (monocot and dicot). Mutagenized curvatures were constructed and confirmed to have disrupted architecture by gel electrophoresis and atomic force microscopy. Relatively small amounts but light-responsive transcripts of psbA2 were observed in cyanobacterial transformants harboring the mutagenized curvature under light/dark and light/high-light conditions. This shows that the curvature is important for basal transcription. In vitro primer extension and DNA mobility shift assay revealed that factors which might bind to the region upstream from the bending center contribute to the effective basal transcription of psbA2.