Perivascular Multipotent Progenitor Cells in Human Organs
Perivascular Multipotent Progenitor Cells in Human Organs
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DOI：
10.1111/j.1749-6632.2009.04967.x
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发表时间：
2009-01-01
期刊：
影响因子：
--
通讯作者：
Peault, Bruno
中科院分区：
文献类型：
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作者：
Crisan, Mihaela;Chen, Chien-Wen;Peault, Bruno
我们已经鉴定了多个人体器官中表达CD 146、NG 2、PDGF-R β和间充质干细胞标志物（CD 44、CD 73、CD 90、CD 105）的血管周细胞，以及缺乏血液、内皮和肌源性细胞标志物的血管周细胞。从所有组织中纯化的周细胞在培养和体内都是肌源性的，持续长期培养，在此期间它们表达间充质干细胞的标志物，并在克隆水平上表现出成骨、成软骨和成脂肪的潜力。这些结果表明，整个生物体的人毛细血管和微血管壁含有祖细胞储备，这些祖细胞是难以捉摸的间充质干细胞的起源，迄今为止仅在原代组织培养中回顾性地鉴定。
We have identified vascular pericytes in multiple human organs on expression of CD146, NG2, PDGF-R beta, and mesenchymal stem cell markers (CD44, CD73, CD90, CD105) and absence of blood, endothelial, and myogenic cell markers. Pericytes purified from all tissues were myogenic in culture and in vivo, sustained long-term culture during which they expressed markers of mesenchymal stem cells, and exhibited, at the clonal level, osteogenic, chondrogenic, and adipogenic potentials. These results suggest that human capillary and microvessel walls all over the organism harbor a reserve of progenitor cells that are at the origin of the elusive mesenchymal stem cells, so far identified only retrospectively in primary tissue cultures.