Indigoids Biosynthesis from Indole by Two Phenol-Degrading Strains, Pseudomonas sp PI1 and Acinetobacter sp PI2
Indigoids Biosynthesis from Indole by Two Phenol-Degrading Strains, Pseudomonas sp PI1 and Acinetobacter sp PI2
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DOI：
10.1007/s12010-015-1644-9
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发表时间：
2015-07-01
影响因子：
3
通讯作者：
Peng, Xiaojun
中科院分区：
文献类型：
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作者：
Wang, Jing;Zhang, Xuwang;Peng, Xiaojun
本研究从活性污泥中分离到两株苯酚降解菌，分别命名为PI 1和PI 2。根据16 S rRNA基因序列分析，菌株PI 1和PI 2分别鉴定为假单胞菌属（Pseudomonas sp.）和不动杆菌属（Acinetobacter sp.），分别采用液相色谱/飞行时间/质谱（LC/TOF/MS）技术对苯酚降解菌转化吲哚过程中的代谢产物进行了分析。结果表明，两株菌株均能催化生成4种靛蓝类化合物，其主要分子离子（M-H）（-）峰均位于m/z 261.067，分子式为C16 H10 N2 O2，包括靛蓝和紫色产物2-（7-氧代-1H-indol-6（7 H）-ylidene）indolin-3-one。检测到中间体靛红和7-羟基吲哚。据此提出了吲哚合成靛蓝类化合物的可能途径。通过响应面法优化了吲哚转化靛蓝的最佳工艺条件，得到菌株PI 1和PI 2的靛蓝产量分别为11.82 ± 0.30和17.19 ± 0.49 mg/L。本研究为微生物生产靛蓝类化合物提供了潜在的候选者。
In this study, two phenol-degrading bacterial strains, designated as PI1 and PI2, were isolated from activated sludge for the production of indigoids from indole. According to the 16S ribosomal RNA (rRNA) gene sequence analysis, strains PI1 and PI2 were identified as Pseudomonas sp. and Acinetobacter sp., respectively. Liquid chromatography/time-of-flight/mass spectrometry (LC/TOF/MS) was applied to analyze the metabolites during the biotransformation of indole by the phenol-degrading strains. The results indicated that both strains could catalyze the formation of four indigoids with the same prominent molecular ion (M-H)(-) peak at m/z 261.067 and molecular formula of C16H10N2O2, including indigo and a purple product, 2-(7-oxo-1H-indol-6(7H)-ylidene) indolin-3-one. Isatin and 7-hydroxyindole were detected as the intermediates. Thus, the possible pathways for the production of indigoids from indole were proposed. Subsequently, the optimal conditions for the production of indigo from indole were determined using response surface methodology, and 11.82 +/- 0.30 and 17.19 +/- 0.49 mg/L indigo were produced by strains PI1 and PI2, respectively. The present study should provide potential candidates for microbial production of indigoids.