The effects of refreezing on the viscoelastic and tensile properties of ligaments
The effects of refreezing on the viscoelastic and tensile properties of ligaments
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DOI：
10.1016/j.jbiomech.2005.02.012
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发表时间：
2006-01-01
影响因子：
2.4
通讯作者：
Abramowitch, SD
中科院分区：
文献类型：
--
作者：
Moon, DK;Woo, SLY;Abramowitch, SD
韧带的生物力学测试方案可以是广泛的，持续两天或更长时间。在此期间，标本可能需要经历不止一次的冷冻和解冻。因此，本研究的目的是评价复冻油对韧带粘弹性和拉伸性能的影响。以6对兔膝关节的股骨-内侧副韧带-胫骨复合体(FMTC)为研究对象。处死后，每对动物中的一条腿被分配给新鲜组，立即解剖FMTC并准备进行测试。对侧膝关节在-20℃下新鲜冷冻3周，解冻后解剖，再冷冻1周，作为复冻组。用激光测微仪测量内侧副韧带(MCL)的横截面积和形态。在单轴拉伸下进行应力松弛和循环应力松弛试验，然后进行载荷到破坏试验。当MCL的粘弹性行为用准线性粘弹性(QLV)理论描述时，基于我们的样本量，新鲜组和复冻组(p>；=0.07)之间的五个常数(A，B，C，tau(1)和tau(2))没有统计上的显著差异。此外，FMTC的结构性能和MCL的力学性能也被发现在两组之间相似(p>；=0.68)。这些结果表明，仔细地重新冻结标本对所测量的生物力学性能几乎没有影响。(C)2005爱思唯尔有限公司。保留所有权利。
Biomechanical testing protocols for ligaments can be extensive and span two or more days. During this time, a specimen may have to undergo more than one cycle of freezing and thawing. Thus, the objective of this study was to evaluate the effects of refreezing oil the viscoelastic and tensile properties of ligaments. The femur-medial collateral ligament-tibia complexes (FMTC) from six pairs of rabbit knees were used for this study. Following sacrifice, one leg in each pair was assigned to the fresh group and the FMTC was immediately dissected and prepared for testing. The contralateral knees were fresh-frozen at -20 degrees C for 3 weeks, thawed, dissected and then refrozen for one additional week before being tested as the refrozen group. The cross-sectional area and shape of the medial collateral ligament (MCL) was measured using a laser micrometer system. Stress relaxation and cyclic stress-relaxation tests in uniaxial tension were performed followed by a load to failure test. When the viscoelastic behavior of the MCL was described by the quasi-linear viscoclastic (QLV) theory, no statistically significant differences could be detected for the five constants (A, B, C, tau(1), and tau(2)) between the fresh and refrozen groups (p >= 0.07) based on our sample size. In addition, the structural properties of the FMTCs and the mechanical properties of the MCLs were also found to be similar between the two groups (p >= 0.68). These results suggest that careful refreezing of the specimens had little or no effect oil the biomechanical properties measured. (c) 2005 Elsevier Ltd. All rights reserved.