Conservation of substrate recognition mechanisms by tRNA splicing endonucleases
Conservation of substrate recognition mechanisms by tRNA splicing endonucleases
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DOI：
10.1126/science.280.5361.284
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发表时间：
1998-04-10
期刊：
影响因子：
56.9
通讯作者：
Tocchini-Valentini, GP
中科院分区：
文献类型：
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作者：
Fabbri, S;Fruscoloni, P;Tocchini-Valentini, GP
在真核生物和古生物中，转移RNA(TRNA)剪接的准确性对于功能tRNAs的形成以及基因表达都是至关重要的。识别tRNA前体(Pre-tRNA)的特异性存在于内切酶中，它通过产生两个独立的内切酶来去除内含子。尽管真核生物和古生物酶似乎使用前tRNA的不同特征来确定切割位点，但对包含真核生物和古生物序列的杂交前tRNA底物的分析表明，真核生物酶保留了使用古生物识别信号的能力。这一结果表明，蛋白质识别Pre-tRNA可能存在共同的祖先机制。
Accuracy in transfer RNA (tRNA) splicing is essential for the formation of functional tRNAs, and hence for gene expression, in both Eukaryotes and Archaea. The specificity for recognition of the tRNA precursor (pre-tRNA) resides in the endonuclease, which removes the intron by making two independent endonucleolytic cleavages. Although the eukaryal and archaeal enzymes appear to use different features of pre-tRNAs to determine the sites of cleavage, analysis of hybrid pre-tRNA substrates containing eukaryal and archaeal sequences, described here, reveals that the eukaryal enzyme retains the ability to use the archaeal recognition signals. This result indicates that there may be a common ancestral mechanism for recognition of pre-tRNA by proteins.