Rapid gene cloning using terminator primers and modular vectors
Rapid gene cloning using terminator primers and modular vectors
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DOI：
10.1093/nar/gnf094
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发表时间：
2002-09-15
影响因子：
14.9
通讯作者：
Jarrell, KA
中科院分区：
文献类型：
--
作者：
Donahue, WF;Turczyk, BM;Jarrell, KA
我们试图通过利用遗传信息的模块化性质来创造有用的生物学多样性。在本报告中，我们描述了侧重于质粒克隆向量的模块化性质的实验。细菌质粒是模块化实体，由复制，可选标记和其他组件组成。我们描述了一种新的与连接无关的克隆方法，该方法允许从组件模块中快速和无缝组装向量。我们进一步证明，基因克隆可以通过模块化矢量的组装同时完成。这种方法提供了相当大的灵活性，因为它允许将基因克隆到自定义组装的模块化向量中。
We seek to create useful biological diversity by exploiting the modular nature of genetic information. In this report we describe experiments that focus on the modular nature of plasmid cloning vectors. Bacterial plasmids are modular entities composed of origins of replication, selectable markers and other components. We describe a new ligation-independent cloning method that allows for rapid and seamless assembly of vectors from component modules. We further demonstrate that gene cloning can be accomplished simultaneously with assembly of a modular vector. This approach provides considerable flexibility as it allows for 'menu driven' cloning of genes into custom assembled modular vectors.