Multinucleated cells formed on calcified dentine from mouse bone marrow cells treated with 1α,25‐dihydroxyvitamin D3 have ruffled borders and resorb dentine
Multinucleated cells formed on calcified dentine from mouse bone marrow cells treated with 1α,25‐dihydroxyvitamin D3 have ruffled borders and resorb dentine
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用 1α,25-二羟基维生素 D3 处理的小鼠骨髓细胞在钙化牙本质上形成的多核细胞具有褶皱边界并吸收牙本质
DOI：
10.1002/ar.1092240307
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
T. Suda
中科院分区：
文献类型：
--
作者：
T. Sasaki;N. Takahashi;S. Higashi;T. Suda
破骨细胞样多核细胞由小鼠骨髓单核细胞形成，并通过透射电子显微镜比较它们在盖玻片和钙化牙本质切片上的形态。向盖玻片上培养的骨髓细胞中添加 1α,25-二羟基维生素 D3 [1α,25(OH)2D3] 可在 8 天内极大地刺激多核细胞的形成。这些多核细胞具有破骨细胞的细胞学特征（丰富的多形性线粒体、大量的液泡和溶酶体、许多堆叠的高尔基膜和广泛的小管系统），但它们既没有褶皱的边界，也没有清晰的区域。多核细胞似乎是单核祖细胞直接融合的结果，其结构特征与多核细胞相似。与分离的破骨细胞一样，多核细胞及其前体在内质网和溶酶体的池中对酒石酸抗性酸性磷酸酶（TRACP）表现出强烈的反应。在 1α,25(OH)2D3 存在下由肺泡巨噬细胞形成的多核细胞对于 TRACP 反应完全呈阴性。当骨髓细胞在 1α,25(OH)2D3 存在的情况下在牙本质切片上培养时，一些多核细胞位于牙本质表面的浅吸收陷窝中，并形成特征性的褶皱边界和透明区域。皱褶边界的狭窄细胞外空间、邻近的苍白内吞空泡和位于多核细胞核周细胞质中的深色溶酶体含有大量吸收陷窝中的磷灰石晶体。这些结果表明（1）用1α,25（OH）2D3处理的小鼠骨髓细胞在盖玻片上形成的多核细胞表现出破骨细胞形态的非功能性基本特征，（2）多核细胞分化为功能性破骨细胞需要钙化牙本质的某些成分。
Osteoclast‐like multinucleated cells were formed from mouse bone marrow mononuclear cells, and their morphology on coverslips and on calcified dentine slices was compared by means of transmission electron microscopy. Addition of 1α,25‐dihydroxyvitamin D3 [1α,25(OH)2D3] to bone marrow cells cultured on coverslips greatly stimulated the formation of multinucleated cells within 8 days. These multinucleated cells had the cytological features of osteoclasts (abundant pleomorphic mitochondria, a large number of vacuoles and lysosomes, many stacks of Golgi membranes, and an extensive canalicular system), but they developed neither ruffled borders nor clear zones. The multinucleated cells appeared to result from direct fusion of mononuclear progenitor cells, whose structural features were similar to those of multinucleated cells. Like isolated osteoclasts, both multinucleated cells and their precursors exhibited an intense reaction for tartrate‐resistant acid phosphatase (TRACP) in the cisterns of endoplasmic reticulum and lysosomes. Multinucleated cells formed from alveolar macrophages in the presence of 1α,25(OH)2D3 were totally negative for TRACP reaction. When marrow cells were cultured on dentine slices in the presence of 1α,25(OH)2D3, some of the multinucleated cells were located in the shallow resorption lacunae of dentine surfaces, and they developed the characteristic ruffled borders and clear zones. The narrow extracellular spaces of the ruffled borders, the adjacent pale endocytotic vacuoles, and the dark lysosomes located in the perinuclear cytoplasm of the multinucleated cells contained numerous apatite crystals delete in resorption lacunae. These results indicate that (1) the multinucleated cells formed on coverslips from mouse marrow cells treated with 1α,25(OH)2D3 exhibit non‐functional basic features of osteoclast morphology, and (2) differentiation of the multinucleated cells into functional osteoclasts requires some components of calcified dentine.
破骨细胞对复杂碳水化合物的细胞外和细胞内消化。
DOI：
--
发表时间：
1982
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Takagi,M;Parmley,RT;Toda,Y;Denys,FR
通讯作者：
Denys,FR