Mucosal immunization with a replication-deficient adenovirus vector expressing murine cytomegalovirus glycoprotein B induces mucosal and systemic immunity.
Mucosal immunization with a replication-deficient adenovirus vector expressing murine cytomegalovirus glycoprotein B induces mucosal and systemic immunity.
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DOI：
10.1016/s0264-410x(03)00037-9
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发表时间：
2003-06
期刊：
影响因子：
5.5
通讯作者：
J. Shanley;Carol A. Wu
中科院分区：
文献类型：
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作者：
J. Shanley;Carol A. Wu
鼠巨细胞病毒 (MCMV) 糖蛋白 B (gB) 基因在腺病毒复制缺陷型载体中表达。这种病毒被命名为 Ad-gB，用于通过鼻内 (i.n.) 途径对 BALB/c 和 B6 小鼠进行免疫，以诱导免疫反应。初次免疫后，100%接种者的血清以及支气管肺泡灌洗液、粪便悬浮液和阴道冲洗液中均检测到抗体。与对照组相比，用 105 或 103 斑块形成单位 (PFU) 的 MCMV 鼻内攻击 10 天后，接种者肺和唾液腺的病毒滴度显着降低。初次免疫后 30 天，疫苗接种者再次接触 Ad-gB 会显着增强血清和粘膜抗体反应，并进一步降低肺和唾液腺中的 MCMV 滴度。诱导对特定基因产物的全身和粘膜免疫反应的能力对于减少 CMV 感染跨粘膜表面的水平传播和改变宿主对 CMV 的免疫力可能很重要。
The murine cytomegalovirus (MCMV) glycoprotein B (gB) gene was expressed in an adenovirus replication-deficient vector. This virus, designated Ad-gB, was used to immunize BALB/c and B6 mice by the intranasal (i.n.) route to induce an immune response. Following primary immunization, antibody was detected in serum of 100% of vaccinees, as well as the bronchoalveolar lavage, fecal suspensions and vaginal washings. The viral titer of lung and salivary gland of vaccinees 10 days after intranasal challenge with MCMV at 105or 103plaque forming units (PFU) were significantly reduced compared to controls. Re-exposure of vaccinees to Ad-gB 30 days after primary immunization induced a remarkable boost of serum and mucosal antibody responses and further reduction of MCMV titers in the lung and salivary glands. The ability to induce both a systemic and mucosal immune response to a specific gene product may be important in reducing horizontal transmission of CMV infections across mucosal surfaces and in altering host immunity to CMV.