Requirement of ZO-1 for the formation of belt-like adherens junctions during epithelial cell polarization.
Requirement of ZO-1 for the formation of belt-like adherens junctions during epithelial cell polarization.
复制标题
DOI：
10.1083/jcb.200612080
复制
发表时间：
2007-03-12
期刊：
影响因子：
--
通讯作者：
Tsukita S
中科院分区：
文献类型：
--
作者：
Ikenouchi J;Umeda K;Tsukita S;Furuse M;Tsukita S
原始粘附连接（AJs）如何在上皮极化过程中演变为空间分离的带状AJs和紧密连接（TJs）的分子机制尚未完全了解。以前，我们报道了ZO-1/ZO-2缺陷培养的上皮细胞（1[ko]/2[kd]细胞）的建立，其完全缺乏TJ。在本研究中，我们发现，带状AJs的形成显着延迟在1（ko）/2（kd）细胞在上皮极化。在1（ko）/2（kd）细胞中，原始AJ形成时Rac 1的活化严重受损。我们的数据表明，ZO-1不仅在TJ形成中起着至关重要的作用，而且通过Rac 1激活在从“成纤维细胞”AJs到带状“极化上皮”AJs的转化中也起着至关重要的作用。此外，为了检查ZO-1本身是否分别介导带状AJ和TJ形成，我们对ZO-1进行了突变分析。带状AJ和TJ形成之间对ZO-1的要求不同。我们建议，ZO-1是直接参与建立两个不同的连接域，带状AJs和TJ，在上皮极化。
The molecular mechanisms of how primordial adherens junctions (AJs) evolve into spatially separated belt-like AJs and tight junctions (TJs) during epithelial polarization are not well understood. Previously, we reported the establishment of ZO-1/ZO-2–deficient cultured epithelial cells (1[ko]/2[kd] cells), which lacked TJs completely. In the present study, we found that the formation of belt-like AJs was significantly delayed in 1(ko)/2(kd) cells during epithelial polarization. The activation of Rac1 upon primordial AJ formation is severely impaired in 1(ko)/2(kd) cells. Our data indicate that ZO-1 plays crucial roles not only in TJ formation, but also in the conversion from “fibroblastic” AJs to belt-like “polarized epithelial” AJs through Rac1 activation. Furthermore, to examine whether ZO-1 itself mediate belt-like AJ and TJ formation, respectively, we performed a mutational analysis of ZO-1. The requirement for ZO-1 differs between belt-like AJ and TJ formation. We propose that ZO-1 is directly involved in the establishment of two distinct junctional domains, belt-like AJs and TJs, during epithelial polarization.
登录
查看更多内容
DOI：
10.1083/jcb.121.3.491
发表时间：
1993-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Itoh M;Nagafuchi A;Yonemura S;Kitani-Yasuda T;Tsukita S;Tsukita S
通讯作者：
Tsukita S
DOI：
10.1083/jcb.200510043
发表时间：
2005-12-19
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ikenouchi J;Furuse M;Furuse K;Sasaki H;Tsukita S;Tsukita S
通讯作者：
Tsukita S
影响因子：
64.5
作者：
Vasioukhin, V;Bauer, C;Fuchs, E
通讯作者：
Fuchs, E
DOI：
10.1083/jcb.107.4.1575
发表时间：
1988-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gumbiner B;Stevenson B;Grimaldi A
通讯作者：
Grimaldi A
影响因子：
4.8
作者：
Masuko, N;Makino, K;Saya, H
通讯作者：
Saya, H