MORTALITY IN ADULT TSETSE, GLOSSINA-MORSITANS MORSITANS, CAUSED BY ENTOMOPATHOGENIC BACTERIA
MORTALITY IN ADULT TSETSE, GLOSSINA-MORSITANS MORSITANS, CAUSED BY ENTOMOPATHOGENIC BACTERIA
复制标题
DOI：
10.1016/0022-2011(89)90136-5
复制
发表时间：
1989-07-01
影响因子：
3.4
通讯作者：
DARJI, N
中科院分区：
文献类型：
--
作者：
KAAYA, GP;DARJI, N
测定了铜绿假单胞菌、粘质沙雷菌、球形芽孢杆菌、蜡样芽孢杆菌、苏云金芽孢杆菌H-14、苏云金芽孢杆菌1、苏云金芽孢杆菌5、苏云金芽孢杆菌insraelensis和雷特氏芽孢杆菌致成年采采蝇的死亡率。在家兔皮肤上涂布细菌，采采蝇在污染区只采食一次，采食铜绿假单胞菌（P . aeruginosa）和粘质假单胞菌（S. marcescens）的采采蝇在采食后8 d的死亡率显著高于对照组（P < 0.01）。当采采蝇第二次在被污染的皮肤上取食时，苏云金芽孢杆菌1和雷氏假单胞菌数量增加。然而，使用这种涂片技术，死亡率一般不显著。在人工膜饲养实验中，使用低浓度的细菌(-106。除苏云金芽孢杆菌H-14致死率为59%外，其余菌株致死率均较低。然而，在该浓度下，铜绿假单胞菌、粘质假单胞菌、蜡样假单胞菌和雷氏假单胞菌的死亡率极显著（P < 0.01）（64-96%）。当使用较高的细菌浓度（107/ml）时，除球形芽孢杆菌外，所有被测细菌的死亡率都很高，在70%至98%之间。因此，死亡率取决于细菌的种类、摄入的剂量和摄入后的时间。
Mortality in adult tsetse, Glossina morsitans morsitans, caused by Pseudomonas aeruginosa, Serratia marcescens, Bacillus sphaericus, Bacillus cereus, Bacillus thuringienss H-14, B. thuringiensis 1, B. thuringiensis 5, B. thuringiensis var. insraelensis, and Providentia rettgeri was determined. When bacteria were smeared on rabbit skin and tsetse allowed to feed only once on the contaminated area, mortality 8 days postingestion was significantly higher (P < 0.01) in tsetse fed on P. aeruginosa, S. marcescens. B. thuringiensis 1, and P. rettgeri was increased when tsetse were allowed to feed for the second time on the contaminated skin. With this smear technique, however, mortalities were generally not remarkable. In artificial membrane feeding experiments using low concentrations of bacteria (-106.ml of blood), the B. thuringiensis strains caused low mortality, except B. thuringiensis H-14, which caused 59% mortality. However, at this concentration, P. aeruginosa, S. marcescens, B. cereus, and P. rettgeri caused highly significant (P < 0.01) mortality (64-96%). When higher concentrations of bacteria (107/ml) were used, all the bacteria tested, except B. sphaericus, caused high mortality ranging from 70 to 98%. Thus, mortality depended on the species of bacteria, the dose ingested, and time postingestion.