Oxidative stress and antioxidants in stress and recovery of cold-hardy insects
Oxidative stress and antioxidants in stress and recovery of cold-hardy insects
复制标题
DOI：
10.1016/s0965-1748(97)00070-2
复制
发表时间：
1998-01-01
影响因子：
3.8
通讯作者：
Storey, KB
中科院分区：
文献类型：
--
作者：
Joanisse, DR;Storey, KB
在冷冻（-14 ° C，24小时）、缺氧（在N-2气体下，24小时）期间以及从这些应激恢复时，研究了耐寒一枝黄花虫瘿昆虫的幼虫中的抗氧化剂、促氧化剂以及氧化应激和损伤。虽然氧化型谷胱甘肽水平在冷冻过程中增加，没有氧化损伤后，从冷冻和缺氧恢复耐冷冻的Eurosta solidaginis。除过氧化氢酶活性在冷冻过程中逐渐增加，在3.5 ℃恢复30 min后达到峰值，为对照值的147%外，抗氧化酶活性没有变化。黄嘌呤氧化酶活性在E.团结一致缺氧和恢复在避免冷冻的情况下也没有导致氧化损伤，尽管促氧化剂黄嘌呤氧化酶略有增加（从12.5%到18%的总黄嘌呤脱氢酶+黄嘌呤氧化酶）的观察，在这个物种中没有观察到抗氧化酶活性的变化，无论是在缺氧期间还是在有氧恢复后，这些幼虫的能力，以限制在冷冻或缺氧暴露过程中的氧再灌注过程中产生的氧化损伤，建议发挥重要的作用，在他们的能力，以生存这些压力。(C)1998爱思唯尔科技有限公司版权所有。
Antioxidants, pro-oxidants, and oxidative stress and damage were studied in larvae of cold-hardy goldenrod gall insects during freezing (-14 degrees C, 24 h), anoxia (under N-2 gas, 24 h), and upon recovery from these stresses. Although oxidized glutathione levels increased during freezing, no oxidative damage was seen upon recovery from freezing and anoxia in freezing tolerant Eurosta solidaginis. Antioxidant enzyme activities were unchanged except for catalase activity which increased gradually during freezing to peak at 147% of control values after 30 min of recovery at 3.5 degrees C. Xanthine oxidase activity was undetected in E. solidaginis. Anoxia and recovery in freeze avoiding Epiblema scudderiana also failed to result in oxidative damage, although a slight increase in the pro-oxidant xanthine oxidase (from 12.5 to 18% of total xanthine dehydrogenase + xanthine oxidase) was observed, No changes in antioxidant enzyme activities were observed in this species either during anoxia or upon aerobic recovery, The ability of these larvae to limit oxidative damage arising during oxygen reperfusion during freezing-or anoxia-exposures is suggested to play an important role in their capacity to survive these stresses. (C) 1998 Elsevier Science Ltd. All rights reserved.