Serotonergic systems associated with arousal and vigilance behaviors following administration of anxiogenic drugs
Serotonergic systems associated with arousal and vigilance behaviors following administration of anxiogenic drugs
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DOI：
10.1016/j.neuroscience.2005.03.025
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发表时间：
2005-01-01
期刊：
影响因子：
3.3
通讯作者：
Lowry, CA
中科院分区：
文献类型：
--
作者：
Abrams, JK;Johnson, PL;Lowry, CA
5-羟色胺能系统在调节行为唤醒中起重要作用，包括与焦虑状态相关的行为唤醒和警觉。为了进一步了解与焦虑状态增加相关的神经系统，我们采用c-Fos和色氨酸羟化酶双重免疫组织化学染色结合大鼠中缝背核（DR）的地形分析，研究了多种致焦虑药物对地形组织的多巴胺能神经元亚群的影响。具有不同药理学特性的促肾上腺皮质激素药物，包括腺苷受体拮抗剂咖啡因、5-羟色胺5-HT 2A/2C受体激动剂间氯苯基哌嗪（mCPP）、α（2）-肾上腺素受体拮抗剂育亨宾和苯二氮卓类受体部分反向激动剂N-甲基-β-咔啉-3-甲酰胺（FG-7142），可诱导与焦虑状态增加一致的行为觉醒和警戒行为增加。此外，这些抗焦虑药物，不包括育亨宾，对DR的中间和尾部、背侧亚部内解剖学定义的多巴胺能神经元亚群具有会聚作用。高分辨率地形分析显示，在中喙尾水平，咖啡因和FG-7142对仅限于先前未定义区域的多巴胺能神经元中的c-Fos表达具有会聚作用，我们将其命名为中缝背核背侧部壳区（DRDSh），它与中缝背核背侧部、中缝背核腹侧部（DRV）和中缝背核腹外侧部（DRVL）之间的解剖边界重叠。逆行追踪方法显示，DRDSH包含大量的神经元投射到基底外侧杏仁核，重要的情绪评估和焦虑相关的生理和行为反应的调制前脑结构。这些研究结果共同支持的假设，有一个功能性的地形组织在DR和一致的假设，即抗焦虑药物有选择性的行动上的一个亚群的肾上腺素能神经元投射到一个分布式的中央自主和情绪运动控制系统调节焦虑状态和焦虑相关的生理和行为反应。(c)2005由Elsevier Ltd代表IBRO出版。
Serotonergic systems play important roles in modulating behavioral arousal, including behavioral arousal and vigilance associated with anxiety states. To further our understanding of the neural systems associated with increases in anxiety states, we investigated the effects of multiple anxiogenic drugs on topographically organized subpopulations of serotonergic neurons using double immunohistochemical staining for c-Fos and tryptophan hydroxylase combined with topographical analysis of the rat dorsal raphe nucleus (DR). Anxiogenic drugs with diverse pharmacological properties including the adenosine receptor antagonist caffeine, the serotonin 5-HT2A/2C receptor agonist m-chlorophenyl piperazine (mCPP), the alpha(2)-adrenoreceptor antagonist yohimbine, and the benzodiazepine receptor partial inverse agonist N-methyl-beta-carboline-3-carboxamide (FG-7142) induced increases in behavioral arousal and vigilance behaviors consistent with an increase in anxiety state. In addition, these anxiogenic drugs, excluding yohimbine, had convergent actions on an anatomically-defined subset of serotonergic neurons within the middle and caudal, dorsal subdivision of the DR. High resolution topographical analysis revealed that at the mid-rostrocaudal level, caffeine and FG-7142 had convergent effects on c-Fos expression in serotonergic neurons that were restricted to a previously undefined region, which we have named the shell region of the dorsal part of the dorsal raphe nucleus (DRDSh), that overlaps the anatomical border between the dorsal part of the dorsal raphe nucleus, the ventral part of the dorsal raphe nucleus (DRV), and the ventrolateral part of the dorsal raphe nucleus (DRVL). Retrograde tracing methods revealed that DRDSh contains large numbers of neurons projecting to the basolateral amygdaloid nucleus, a forebrain structure important for emotional appraisal and modulation of anxiety-related physiological and behavioral responses. Together these findings support the hypothesis that there is a functional topographical organization in the DR and are consistent with the hypothesis that anxiogenic drugs have selective actions on a subpopulation of serotonergic neurons projecting to a distributed central autonomic and emotional motor control system regulating anxiety states and anxiety-related physiological and behavioral responses. (c) 2005 Published by Elsevier Ltd on behalf of IBRO.