Fractionation of apple procyanidins by size-exclusion chromatography
Fractionation of apple procyanidins by size-exclusion chromatography
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DOI：
10.1016/s0021-9673(99)00684-6
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发表时间：
1999-09-03
影响因子：
4.1
通讯作者：
Nagata, T
中科院分区：
文献类型：
--
作者：
Yanagida, A;Kanda, T;Nagata, T
采用TSKgel Toyopolysis HW-40 F分子筛对苹果原花青素的低聚物进行了分离。最好的分离，得到使用移动的流动相的丙酮-8 M尿素（6：4;调整到pH 2）在1.0 ml/min的流速。在该色谱系统中，使用8 M尿素在移动的相导致在分子筛的效果没有任何表面亲和凝胶珠和矢车菊苷配质分子之间的相互作用。通过反相高效液相色谱法和飞行时间质谱法检查获得的每个馏分。原花青素的洗脱顺序与其聚合度一致。(C)1999 Elsevier Science B. V.保留所有权利。
Oligomeric constituents of apple procyanidins were fractionated by size-exclusion chromatography using a TSKgel Toyopearl HW-40F column. The best separation was obtained using a mobile phase of acetone-8 M urea (6:4; adjusted to pH 2) at a flow-rate of 1.0 ml/min. In this chromatographic system, the use of 8 M urea in the mobile phase resulted in a molecular sieve effect without any surface affinity interaction between the gel beads and the procyanidin molecules. Each fraction obtained was examined by reversed-phase high-performance liquid chromatography and time-of-flight mass spectrometry. The order of elution of the procyanidins from the column was coincident with their degree of polymerization. (C) 1999 Elsevier Science B.V. All rights reserved.