T gamma (T gamma) cells suppress growth of erythroid colony-forming units in vitro in the pure red cell aplasia of B-cell chronic lymphocytic leukemia.
T gamma (T gamma) cells suppress growth of erythroid colony-forming units in vitro in the pure red cell aplasia of B-cell chronic lymphocytic leukemia.
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T γ (T γ) 细胞在体外抑制 B 细胞慢性淋巴细胞白血病纯红细胞再生障碍性红细胞集落形成单位的生长。
DOI：
10.1172/jci110713
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Farley,PC
中科院分区：
文献类型：
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作者：
Mangan,KF;Chikkappa,G;Farley,PC
体外研究在两例b细胞慢性淋巴细胞白血病患者中进行，他们发展为纯红细胞发育不全（CLL-PRCA）。在红细胞发育不全的活跃期，红细胞集落形成单位（CFU-E）的数量明显减少。这些患者未分离的血清或分离的IgG片段均未损害来自自体或异体骨髓的CFU-E增殖。这些患者骨髓抽吸液中T淋巴细胞数量增加。对这些T细胞的分析表明，分别有90%和35%的T细胞具有IgG的Fc受体。通过e - rossetting技术去除T细胞可使CLL-PRCA中CFU-E的生长增加10倍。正常骨髓的类似处理没有引起类似的CFU-E生长增强。在CLL-PRCA活性阶段获得的骨髓T细胞或T γ细胞共同培养抑制来自自体或异体骨髓的CFU-E生长。在实现药物诱导的PRCA缓解后，骨髓T细胞不再具有抑制性。相比之下，BFU-E（红细胞爆发形成单位）或扩散室中的粒细胞增殖不受CLL-PRCA T细胞的抑制。这些发现表明，在b细胞CLL中，PRCA的发展可能是由于T细胞抑制CFU-E增殖。
In vitro studies were performed in two patients with B-cell chronic lymphocytic leukemia who developed pure red cell aplasia (CLL-PRCA). During the active phase of their red cell aplasia, there was a marked reduction in the numbers of erythroid colony-forming units (CFU-E). Unfractionated sera or separated IgG fractions from these patients did not impair CFU-E proliferation from either autologous or allogeneic marrows. Increased numbers of T lymphocytes were present in marrow aspirates of these patients. Analysis of these T cells indicated that 90 and 35%, respectively, bore Fc receptors for IgG (T gamma cells). Removal of T cells by E-rosetting techniques augmented CFU-E growth in CLL-PRCA 10-fold. Similar treatment of normal marrows did not cause similar enhanced growth of CFU-E. Co-cultures of marrow T cells or T gamma cells obtained during the active phase of CLL-PRCA suppressed CFU-E growth from autologous or allogeneic marrows. After achieving drug-induced remission of the PRCA, marrow T cells were no longer inhibitory. In contrast, BFU-E (erythroid burst-forming units) or granulocyte proliferation in diffusion chambers were not suppressed by CLL-PRCA T cells. These findings suggest that the development of PRCA in B-cell CLL may result from suppression of CFU-E proliferation by T gamma cells.