Measurement of tumor oxygenation: A comparison between polarographic needle electrodes and a time-resolved luminescence-based optical sensor
Measurement of tumor oxygenation: A comparison between polarographic needle electrodes and a time-resolved luminescence-based optical sensor
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DOI：
10.2307/3579340
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发表时间：
1997-03-01
影响因子：
3.4
通讯作者：
Chaplin, DJ
中科院分区：
文献类型：
--
作者：
Collingridge, DR;Young, WK;Chaplin, DJ
一种新的氧传感器，它不依赖于电化学还原已被用于测量的小鼠肉瘤F的氧合在与现有的极谱电极，是可商购的比较研究。原型发光传感器产生的氧气分布与使用pO（2）直方图的读数相当。使用Eppendorf pO（2）组织图和发光光纤传感器检测到的pO（2）小于5 mm Hg的区域百分比分别为79和75。这些值与该肿瘤中67%的放射生物学低氧分数相一致。与光学传感器（50%）相比，极谱法检测到更多pO_1 ≤ 2.5 mm Hg的区域（69%）（P < 0.05）。这可能反映了传感装置氧气使用的差异。这一初步评估表明了基于光纤的氧气监测系统的潜力。这样的系统应该具有几个优点，包括监测给定微区域中的时间氧变化和与NMR程序一起使用。(C)1997年，辐射研究协会。
A novel oxygen sensor which does not rely on electrochemical reduction has been used to measure the oxygenation of the murine sarcoma F in a comparative study with an existing polarographic electrode that is available commercially. The prototype luminescence sensor yielded an oxygen distribution comparable with readings made using a pO(2) histograph. The percentage of regions detected that had a pO(2) less than 5 mm Hg was 79 and 75 using the Eppendorf pO(2) histograph and the luminescence fiber optic sensor, respectively. These values were compatible with a measured radiobiologically hypoxic fraction of 67% in this tumor. The polarographic method detected more regions with a pO, of 2.5 mm Hg or less (69%) compared with the optical sensor (50%) (P < 0.05). This could reflect differences in the oxygen use of the sensing devices. This initial assessment indicates the potential of a fiber-optic-based oxygen-monitoring system. Such a system should have several advantages including monitoring temporal oxygen changes in a given microregion and use with NMR procedures. (C) 1997 by Radiation Research Society.