INFLUENCE OF PHENOLS ON GROWTH AND MEMBRANE-PERMEABILITY OF FREE AND IMMOBILIZED ESCHERICHIA-COLI
INFLUENCE OF PHENOLS ON GROWTH AND MEMBRANE-PERMEABILITY OF FREE AND IMMOBILIZED ESCHERICHIA-COLI
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DOI：
10.1128/aem.57.4.1213-1217.1991
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发表时间：
1991-04-01
影响因子：
4.4
通讯作者：
REHM, HJ
中科院分区：
文献类型：
--
作者：
HEIPIEPER, HJ;KEWELOH, H;REHM, HJ
研究了不同酚类化合物对大肠杆菌K-12指数生长的抑制作用。 海藻酸钙包埋的细胞比悬浮培养的细胞表现出更大的耐受性。 固定化细胞的生长抑制程度取决于在凝胶基质中的生长时间。 在加入抑菌浓度的苯酚或4-氯苯酚后，引起了代谢产物如ATP和K+离子的剂量依赖性流出。 提供葡萄糖作为能量底物，在低苯酚浓度下观察到K+离子的再积累。 恢复膜梯度的K+总是在继续生长的毒性化合物的存在下。 与游离细胞相比，加入4-氯苯酚后，固定化细胞在海藻酸钠中生长时，阳离子损失较小。 在较高浓度的污染物与截留细胞比与自由细胞的梯度重建观察。 对应于耐受性的增加，在以固定形式生长更长时间的细胞中膜损伤减少。 这些数据提供了一个机械解释的保护固定化微生物从酚类溶剂。 这些数据表明，在这些污染物的毒性中，膜是重要的细胞成分。
The inhibition of the exponential growth of Escherichia coli K-12 by different phenolic compounds was examined. Cells entrapped in calcium alginate showed a greater tolerance than cells grown in suspension. The extent of inhibition of growth of the immobilized cells depended on the period of growth in the gel matrix. After the addition of bacteriostatic concentrations of phenol or 4-chlorophenol, a dose-dependent efflux of metabolites such as ATP and of K+ ions was elicited. Provided that glucose was supplied as an energy substrate, a reaccumulation of K+ ions at low phenol concentrations was observed. The restoration of the membrane gradient for K+ always preceded the continuation of growth in the presence of the toxic compounds. Compared with free cells, those cells immobilized and grown in alginate suffered a smaller loss of cations after the addition of 4-chlorophenol. The reestablishment of gradients was observed at higher concentrations of the pollutants with entrapped cells than with free cells. Corresponding to the increase in tolerance, the membrane damage was reduced in cells grown in immobilized form for longer times. These data offer a mechanistic explanation of the protection of immobilized microorganisms from phenolic solvents. The data point to the membrane as an important cell component in the toxicity of these pollutants.