Biosynthesis of the Respiratory Toxin Bongkrekic Acid in the Pathogenic Bacterium Burkholderia gladioli
Biosynthesis of the Respiratory Toxin Bongkrekic Acid in the Pathogenic Bacterium Burkholderia gladioli
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DOI：
10.1016/j.chembiol.2012.07.022
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发表时间：
2012-09-21
影响因子：
--
通讯作者：
Hertweck, Christian
中科院分区：
文献类型：
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作者：
Moebius, Nadine;Ross, Claudia;Hertweck, Christian
Bongkrekic acid（BA）是一种臭名昭著的病原菌唐菖蒲伯克霍尔德氏菌（Burkholderia gladioli）的呼吸道毒素，当用受污染的少孢根霉培养物生产滕佩时，它会导致致命的中毒。B的基因组测序。剑兰致病变种cocovenenans揭示了BA生物合成的遗传基础，并指出在a B中存在一个同源的Bon基因簇。一种从受感染的水稻植株上分离的唐菖蒲菌株。对于B的功能遗传学。唐菖蒲λ Red重组建立。模块化I型聚酮合酶（反式AT PKS）的解剖提供了深入了解复杂的聚酮组装。类异戊二烯样β-分支事件和六电子甲基氧化成羧酸产生独特的支链三羧酸脂肪酸。细胞色素P450单加氧酶BonL的作用通过对突变体中的脱氧米酵菌酸进行结构解析而得到证实。
Bongkrekic acid (BA), an infamous respiratory toxin of the pathogenic bacterium Burkholderia gladioli, causes lethal intoxications when tempe bongkrek is produced with contaminated Rhizopus oligosporus cultures. Genome sequencing of B. gladioli pathovar cocovenenans unveiled the genetic basis for BA biosynthesis, and pointed to a homologous bon gene cluster in a B. gladioli strain from an infected rice plant. For functional genetics in B. gladioli lambda Red recombination was established. Dissection of the modular type I polyketide synthase (a trans-AT PKS) provided insights into complex polyketide assembly. Isoprenoid-like beta-branching events and a six-electron oxidation of a methyl group to a carboxylic acid give rise to the unique branched tricarboxylic fatty acid. The role of the cytochrome P450 monooxygenase, BonL, was proven by structural elucidation of deoxybongkrekic acid from a mutant.