The effect of fasting at different ages on growth and tissue dynamics in the small intestine of the young chick
The effect of fasting at different ages on growth and tissue dynamics in the small intestine of the young chick
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DOI：
10.1079/bjn2001368
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发表时间：
2001-07-01
影响因子：
3.6
通讯作者：
Sklan, D
中科院分区：
文献类型：
--
作者：
Geyra, A;Uni, Z;Sklan, D
孵化雏鸡的小肠在刚孵出的时期经历了快速的发育变化，从蛋黄的内源营养供应过渡到对外源饲料的依赖。由于生产的后勤考虑，这种转变通常在孵化后48小时或更长时间才开始。在这一关键时期，不同时间禁食48小时对小肠发育和肠细胞动力学的影响通过形态学测定和增殖细胞核抗原和5-溴-2-脱氧尿苷染色来检测。禁食的效果与禁食时间和所检查的肠段有关。十二指肠和空肠发育减少，但回肠发育不明显。禁食0 ~ 48 h使十二指肠和空肠隐窝大小、每绒毛隐窝数量、隐窝增殖、绒毛面积和肠细胞迁移率降低。虽然空肠似乎是肠道中最敏感的部分，但在较晚的时间禁食导致的影响较小。生长与隐窝细胞数、绒毛细胞数和节段表面积相关。雏鸡在孵化后超过48小时才开始饲喂的做法可能会抑制后续的发育。
The small intestines of hatching chicks undergo rapid developmental changes in the immediate post-hatch period when the birds are making the transition from endogenous nutrient supply from yolk to dependence on exogenous feed. This transition usually only begins 48 h or more after hatching, owing to logistical considerations of production. The effects of fasting for 48 h at different times during this critical period on small intestinal development and enterocyte dynamics were examined by morphometric determinations and use of staining for proliferative-cell nuclear antigen and 5-bromo-2-deoxyuridine. The effects of fasting were specific to both time of fasting and the intestinal segment examined. Decreased development was found in the duodenum and jejunum, but was less apparent in the ileum. Fasting between 0 and 48 h decreased crypt size in the duodenum and jejunum, the number of crypts per villus, crypt proliferation, villus area and the rate of enterocyte migration. Fasting at later times resulted in smaller effects, although the jejunum appeared to be the most sensitive of the intestinal segments. Growth was correlated with the number of cells in the crypts, the number of cells along the villus and the segment surface area. The common practice whereby feed is first available to chicks more than 48 h post-hatch may depress subsequent development.