A highly sensitive bioelectrochemical toxicity sensor and its evaluation using immediate current attenuation
A highly sensitive bioelectrochemical toxicity sensor and its evaluation using immediate current attenuation
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一种高灵敏生物电化学毒性传感器及其即时电流衰减评估
DOI：
10.1016/j.scitotenv.2020.142646
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发表时间：
2021
影响因子：
9.8
通讯作者：
Wang Xin
中科院分区：
文献类型：
--
作者：
Li Tian;Liao Chengmei;An Jingkun;Zhou Lean;Tian Lili;Zhou Qixing;Li Nan;Wang Xin
电活性生物膜（EAB）传感器因其低成本、广谱性，在水体毒物预警领域显示出巨大的潜力。然而，传统的灵敏度计算强烈依赖于时间和浓度梯度，削弱了传感器的时间效率。此外，灵敏度可以进一步提高，以响应痕量浓度。在这里，形成具有不同底物浓度的EAB传感器以响应范围从1 ppm至50 ppm的不同浓度的甲醛，并诱导即时电流衰减（伊卡）以评估灵敏度。伊卡（~70 s）比可计算灵敏度（CS）和电流衰减（CA）计算的时间更短，不仅实现了痕量浓度的响应，而且提高了传感器的时效性。用0.1 g/L乙酸盐形成的EAB（EAB-0.1）比用1.0 g/L乙酸盐形成的EAB（EAB-1.0）具有高380%的灵敏度，导致对1 ppm甲醛的显著电化学毒性响应。电化学响应系数的测定结果表明，在0.2 ~ 1.0g/L乙酸盐浓度范围内，EAB-0.1的生成量是其生成量的1.5-6.3倍，这与前期研究中所发现的微生物群落和EAB组分有关。实验结果表明，灵敏度的计算可以优化，以反映时间效率和EAB的限制乙酸可以应用于痕量毒物的检测。
Electroactive biofilm (EAB) sensor had shown great potential in the field of early warning of toxicants in water because of the low-cost and broad-spectrum. However, the traditional calculation of sensitivity strongly relied on the time and concentration gradient which weakened time-efficiency of the sensor. Moreover, the sensitivity could be further improved to respond trace concentrations. Here EAB sensors with different substrate concentrations were formed to respond different concentrations formaldehyde ranging from 1 ppm to 50 ppm and immediate current attenuation (ICA) was induced to evaluate the sensitivity. The ICA (~70 s) exhibited a shorter time than that calculated by calculable sensitivity (CS) and current attenuation (CA), which not only achieved the response of trace concentration but also improved the time-efficiency of the sensor. The EAB formed with 0.1 g/L acetate (EAB-0.1) had a 380% higher sensitivity than that formed with 1.0 g/L acetate (EAB-1.0), leading to a significant electrochemical toxicity response to 1 ppm of formaldehyde. The results of electrochemical response coefficient confirmed that EAB-0.1 was 1.5–6.3 times of that formed with acetate from 0.2 to 1.0 g/L, which was related with microbial community and component of EAB as described in our previous study. Our findings demonstrated that calculation of sensitivity could be optimized to reflect time-efficiency and EAB with limit acetate could be applied in trace toxicant detection.