Ki-67 immunocytochemistry in liquid based cervical cytology: useful as an adjunctive tool?
Ki-67 immunocytochemistry in liquid based cervical cytology: useful as an adjunctive tool?
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DOI：
10.1136/jcp.56.9.681
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发表时间：
2003-09-01
影响因子：
3.4
通讯作者：
Bogers, JJ
中科院分区：
文献类型：
--
作者：
Sahebali, S;Depuydt, CE;Bogers, JJ
目的：为了测试Ki-67在筛查环境中检测细胞学病变的能力及其作为人乳头瘤病毒（HPV）感染的替代标志物的用途。方法：对随机选择的147名患者进行液基细胞学、MY 09/MY 11共有聚合酶链反应（PCR）HPV DNA检测、类型特异性PCR和Ki-67免疫细胞化学研究。Ki-67免疫反应细胞/1000细胞在不同的细胞学组的数量比较表明，HSIL组产生了显着较高的平均计数比其他。组HPV-16阳性样本中Ki-67免疫反应细胞/1000个细胞的数量显着高于含有其他高危类型感染的样本。受试者工作特征曲线表明，对于意义不明的非典型鳞状细胞（ASCUS）、低度鳞状上皮内病变（LSIL）和高度鳞状上皮内病变（HSIL），检验准确度（曲线下面积）分别为0.68、0.72和0.86。ASCUS、LSIL和HSIL的95%灵敏度阈值分别为0.07、0.08和0.15 Ki-67免疫阳性细胞/1000个细胞。95%特异性的阈值为1.9 Ki-67免疫阳性细胞/1000 cells。结论：Ki-67免疫细胞化学可应用于液基细胞学检查。与其他技术相比，该试验的准确性和诊断指标良好。作为一组筛查程序的一部分，它可以用作液基细胞学的辅助手段来识别HSIL，并作为HPV-16感染的替代标志物。
Aims: To test the ability of Ki-67 to detect cytological lesions in a screening setting and its use as a surrogate marker of human papillomavirus (HPV) infection.Methods: A study of liquid based cytology, HPV DNA testing by MY09/MY11 consensus polymerase chain reaction (PCR), type specific PCRs, and Ki-67 immunocytochemistry on a randomly selected series of 147 patients.Results: Comparison of the number of Ki-67 immunoreactive cells/1000 cells in the different cytological groups showed that the HSIL group yielded a significantly higher mean count than did the other. groups. The number of Ki-67 immunoreactive cells/1000 cells was significantly higher in HPV-16 positive samples than in samples containing infections with other high risk types. Receiver operating characteristic curves indicated a test accuracy (area under curve) of 0.68, 0.72, and 0.86 for atypical squamous cells of undetermined significance (ASCUS), low grade squamous intraepithelial lesions (LSIL), and high grade squamous intraepithelial lesions (HSIL), respectively. Thresholds for 95% sensitivity were 0.07, 0.08, and 0.15 Ki-67 immunopositive cells/1000 cells for ASCUS, LSIL and HSIL, respectively. The threshold for 95% specificity was 1.9 Ki-67 immunopositive cells/1000 cells.Conclusions: Ki-67 immunocytochemistry can be applied to liquid based cytology. The accuracy and diagnostic indices of the test are good when compared with those of other techniques. As part of a panel of screening procedures, it could be used as an adjunct to liquid based cytology to identify HSIL, and as a surrogate marker of HPV-16 infection.