A SODIUM TRANSPORT INHIBITORY FACTOR IN SALIVA OF PATIENTS WITH CYSTIC FIBROSIS OF PANCREAS
A SODIUM TRANSPORT INHIBITORY FACTOR IN SALIVA OF PATIENTS WITH CYSTIC FIBROSIS OF PANCREAS
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DOI：
10.1203/00006450-196711000-00002
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发表时间：
1967-01-01
影响因子：
3.6
通讯作者：
BENKE, PJ
中科院分区：
文献类型：
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作者：
MANGOS, JA;MCSHERRY, NR;BENKE, PJ
胰腺囊性纤维化症(CFP)患者唾液中Na含量高于正常人唾液。对CFP患者唾液电解质异常可能是由于唾液中存在钠转运抑制因子的假设进行了研究。这样的因素会降低唾液腺对钠的重吸收，并导致最终唾液中钠浓度的增加。采用大鼠腮腺逆行灌注法测定该因子。用生理盐水或正常儿童唾液逆行灌流不影响大鼠腮腺重吸收钠的速率。逆行灌流含生理盐水或CFP患者唾液的哇巴因可显著降低钠重吸收率。CFP患者的唾液中含有一种抑制大鼠腮腺钠转运的因素。患者唾液对牛腮腺、人红细胞和大鼠肾脏膜ATPase制剂的体外活性无影响。这表明，CFP患者的唾液通过抑制ATPase的活性而不是通过抑制ATPase的活性来抑制大鼠腮腺的钠重吸收，ATPase是一种已知参与钠跨生物膜运输的酶。
The saliva of patients with cystic fibrosis of the pancreas (CFP) contains higher concentratioas of Na than the saliva of normal subjects. The hypothesis that the salivary electrolyte abnormality in CFP may be due to a Na transport inhibitory factor (or factors) present in the saliva of these patients was investigated. Such a factor would decrease Na reabsorption in the salivary glands and cause increased Na concentrations in the final saliva. Retrograde perfusion of the rat parotid gland was used to determine this factor. Retrograde perfusion with normal saline or saliva from normal children did not affect the rate of Na reabsorption in the rat parotid gland. Retrograde perfusion with ouabain containing saline or saliva from patients with CFP resulted in a marked decrease of the rate of Na reabsorption. Saliva from patients with CFP contains a factor that causes inhibition of Na transport in the rat parotid gland. The saliva of patients had no effect on the in vitro activity of membrane ATPase preparations obtained from beef parotid, human erythrocytes and rat kidneys. This indicates that the saliva of patients with CFP causes inhibition of Na reabsorption in the rat parotid by an action other than inhibition of the activity of ATPase, an enzyme known to be involved in Na transport across biological membranes.