Browning of Human Subcutaneous Adipose Tissue after Its Transplantation in Nude Mice
Browning of Human Subcutaneous Adipose Tissue after Its Transplantation in Nude Mice
复制标题
人皮下脂肪组织移植裸鼠后的褐变
DOI：
10.1097/prs.0000000000004603
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发表时间：
2018-08-01
影响因子：
3.6
通讯作者：
Yi，Chenggang
中科院分区：
文献类型：
--
作者：
Qiu，Lihong;Zhang，Zhaoxiang;Yi，Chenggang
背景：自体脂肪移植的临床不可预测性部分源于脂肪组织的独特特性。有证据表明，创伤后脂肪重塑为褐化(白色脂肪组织中发育出棕色样脂肪细胞)。关于脂肪移植手术直接和无处不在地损伤脂肪组织，是否会影响脂肪表型向褐变的转变，以前还没有报道。方法：采用吸脂法从女性患者腹部获取人皮下脂肪组织，注射于裸鼠背侧。12周后，取脂肪移植物进行组织学分析。结果：苏木精-伊红染色显示移植物周围可见小的多房性脂肪细胞。与中央脂肪细胞相比，这些脂肪细胞的解偶联蛋白1(一种脂肪褐变特异性标志物)、线粒体蛋白和CD31的染色较高，表明该区域存在棕色样脂肪细胞(即米色脂肪细胞)。此外，免疫荧光染色显示这些米色脂肪细胞可能来源于移植物来源的前体细胞的新生脂肪生成。结论：皮下白色脂肪组织的褐变参与了移植后适应性组织重塑，有助于脂肪组织的修复。
Background: The clinical unpredictability of autologous fat grafting originates partially from the unique characteristics of adipose tissue. Evidence of adipose remodeling toward browning (developing of brown-like adipocytes in white adipose tissue) in response to trauma has been emerging. With regard to fat grafting surgery by which adipose tissue depots are directly and ubiquitously traumatized, whether it affects adipose phenotype change toward browning has not been previously reported. Methods: Human subcutaneous adipose tissues were harvested from the abdominal region of female patients by means of liposuction and were then injected into the dorsal flanks of athymic nude mice. After 12 weeks, fat grafts were harvested and subjected to histologic analysis. Results: Hematoxylin and eosin staining showed the appearance of small multilocular adipocytes in the peripheral region of the grafts. These adipocytes exhibited higher staining for uncoupling protein 1 (a fat browning–specific marker), mitochondrial protein, and CD31 compared with the central ones, indicating the presence of brown-like adipocytes (i.e., beige adipocytes) in this area. Furthermore, immunofluorescence staining demonstrated that these beige adipocytes might be derived from de novo adipogenesis from progenitors of graft origin. Conclusion: Results of this study suggest that browning of subcutaneous white adipose tissue participates in adaptive tissue remodeling following grafting and contributes to adipose tissue repair.