The soybean GmbZIP1 transcription factor enhances multiple abiotic stress tolerances in transgenic plants
The soybean GmbZIP1 transcription factor enhances multiple abiotic stress tolerances in transgenic plants
复制标题
大豆 GmbZIP1 转录因子增强转基因植物的多种非生物胁迫耐受性
DOI：
10.1007/s11103-011-9738-4
复制
发表时间：
2011-04-01
影响因子：
5.1
通讯作者：
Ma, You-Zhi
中科院分区：
文献类型：
--
作者：
Gao, Shi-Qing;Chen, Ming;Ma, You-Zhi
脱落酸 (ABA) 响应元件结合蛋白 (AREB) 是碱性结构域/亮氨酸拉链转录因子，可与植物中 ABA 诱导基因启动子区域的 ABA 响应元件 (ABRE) 结合。从耐盐大豆品种中分离得到一个新的bZIP转录因子基因GmbZIP1，该基因编码438个氨基酸，其保守的bZIP结构域由60个氨基酸组成。铁峰8。Southern印迹显示大豆基因组中仅存在一个拷贝。系统发育分析表明，GmbZIP1属于bZIP家族的AREB亚家族，与AtABF2和OsTRAB1关系最密切。 GmbZIP1的表达受ABA、干旱、高盐和低温高度诱导；而GmbZIP1在不同胁迫条件下在大豆根、茎、叶中均有表达。 GmbZIP1 位于转化洋葱表皮细胞的细胞核内。 GmbZIP1的过度表达增强了转基因植物对ABA的反应，并在胁迫下触发气孔关闭，可能导致转基因植物对多种非生物胁迫（如高盐、低温和干旱）的耐受性提高。此外，GmbZIP1的过表达影响了拟南芥在ABA、干旱和高盐胁迫条件下参与调节气孔关闭的一些ABA或胁迫相关基因的表达。在这些 ABA 或胁迫相关基因的启动子区域检测到一些 AREB 元件，表明 GmbZIP1 调节转基因拟南芥中这些下游基因介导的 ABA 反应或气孔关闭。此外，GmbZIP1还用于改良中国小麦品种BS93的耐旱性状。功能分析表明，GmbZIP1的过表达增强了转基因小麦的耐旱性，并且RT-PCR在转基因小麦中检测到了GmbZIP1的转录本。此外，GmbZIP1过表达并未导致所有转基因植物生长迟缓，这表明GmbZIP1可能是工程作物抗逆性的宝贵遗传资源。
Abscisic acid (ABA)-responsive element binding proteins (AREBs) are basic domain/leucine zipper transcription factors that bind to the ABA-responsive element (ABRE) in the promoter regions of ABA-inducible genes in plants. A novel bZIP transcription factor gene,GmbZIP1, encoding 438 amino acids with a conserved bZIP domain composed of 60 amino acids was isolated from salt-tolerant soybean cv. Tiefeng 8. Southern blotting showed that only one copy was present in the soybean genome. Phylogenetic analyses showed that GmbZIP1 belonged to the AREB subfamily of the bZIP family and was most closely related to AtABF2 and OsTRAB1. The expression ofGmbZIP1was highly induced by ABA, drought, high salt and low temperature; andGmbZIP1was expressed in soybean roots, stems and leaves under different stress conditions. GmbZIP1 was localized inside the nuclei of transformed onion epidermal cells. Overexpression ofGmbZIP1enhanced the responses of transgenic plants to ABA and triggered stomatal closure under stresses, potentially leading to improved tolerances to several abiotic stresses such as high salt, low temperature and drought in transgenic plants. Furthermore, overexpression ofGmbZIP1affected the expression of some ABA or stress-related genes involved in regulating stomatal closure inArabidopsisunder ABA, drought and high salt stress conditions. A few AREB elements were detected in the promoter region of those ABA or stress-related genes, suggesting that GmbZIP1 regulates the ABA response or stomatal closure mediated by those downstream genes in transgenicArabidopsis. Moreover,GmbZIP1was used to improve the drought tolerance trait of Chinese wheat varieties BS93. Functional analysis showed that overexpression ofGmbZIP1enhanced the drought tolerance of transgenic wheat, and transcripts ofGmbZIP1were detected in transgenic wheat using RT-PCR. In addition, GmbZIP1 overexpression did not result in growth retardation in all transgenic plants, suggesting thatGmbZIP1may be a valuable genetic resource for engineering stress tolerance of crops.