Bromodomain proteins GTE9 and GTE11 are essential for specific BT2-mediated sugar and ABA responses in Arabidopsis thaliana
Bromodomain proteins GTE9 and GTE11 are essential for specific BT2-mediated sugar and ABA responses in Arabidopsis thaliana
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DOI：
10.1007/s11103-018-0704-2
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发表时间：
2018-03-01
影响因子：
5.1
通讯作者：
Mandadi, Kranthi K.
中科院分区：
文献类型：
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作者：
Misra, Anjali;McKnight, Thomas D.;Mandadi, Kranthi K.
在拟南芥中，全局转录因子E组蛋白GTE 9和GTE 11与BT 2相互作用，介导阿坝和糖的应答。BT 2是一个BTB结构域蛋白，在拟南芥中调节对各种激素、胁迫和代谢条件的应答。BT 2的缺失导致植物对脱落酸（阿坝）和糖的发芽抑制超敏感。相反，BT 2的过表达导致对阿坝和糖的抗性。在这里，我们报告的BT 2相互作用的合作伙伴GTE 9和GTE 11，溴结构域和全球转录因子E组的末端外结构域蛋白，在BT 2介导的糖和激素的反应的作用。失去功能的突变体，gte 9 -1和gte 11 -1，模仿bt 2 -1无效突变体的反应，发芽的所有三个突变体是超敏感的葡萄糖和阿坝的抑制。在BT 2过表达株系中GTE 9或GTE 11的缺失阻断了对糖和阿坝的抗性，表明GTE 9和GTE 11都是BT 2功能所需的。BT 2和GTE 9的免疫共沉淀表明，这些蛋白质在体内物理相互作用，并推测共同发挥作用，介导对阿坝和糖信号的反应。
Global Transcription Factor Group E proteins GTE9 and GTE11 interact with BT2 to mediate ABA and sugar responses in Arabidopsis thaliana.BT2 is a BTB-domain protein that regulates responses to various hormone, stress and metabolic conditions in Arabidopsis thaliana. Loss of BT2 results in plants that are hypersensitive to inhibition of germination by abscisic acid (ABA) and sugars. Conversely, overexpression of BT2 results in resistance to ABA and sugars. Here, we report the roles of BT2-interacting partners GTE9 and GTE11, bromodomain and extraterminal-domain proteins of Global Transcription Factor Group E, in BT2-mediated responses to sugars and hormones. Loss-of-function mutants, gte9-1 and gte11-1, mimicked the bt2-1-null mutant responses; germination of all three mutants was hypersensitive to inhibition by glucose and ABA. Loss of either GTE9 or GTE11 in a BT2 over-expressing line blocked resistance to sugars and ABA, indicating that both GTE9 and GTE11 were required for BT2 function. Co-immunoprecipitation of BT2 and GTE9 suggested that these proteins physically interact in vivo, and presumably function together to mediate responses to ABA and sugar signals.