In vitro detection of small molecule metabolites excreted from cancer cells using a Tenax TA thin-film microextraction device
In vitro detection of small molecule metabolites excreted from cancer cells using a Tenax TA thin-film microextraction device
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DOI：
10.1016/j.jchromb.2015.04.016
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发表时间：
2015-06-01
影响因子：
3
通讯作者：
Lee, Seung-Woo
中科院分区：
文献类型：
--
作者：
Nozoe, Takuma;Goda, Shigemi;Lee, Seung-Woo
我们开发了一种新的装置，用于体外提取癌细胞分泌的小分子代谢物。浸提装置具有生物相容性并与癌细胞一起孵育，由沉积在圆柱形铝棒表面上的薄Tenax TA膜组成。Tenax TA固相用于从细胞培养样品中直接提取和预浓缩小分子代谢物。使用HeLa细胞培养物对器械制造和代谢物提取进行了优化、测试和验证。将代谢谱与培养基的对照测量值进行比较，能够检测细胞培养物消耗或产生的代谢物。通过GC-MS分析对排泄代谢物进行了初步鉴定和半定量研究。所提出的方法可以是一个有价值的工具，用于表征低挥发性的癌细胞代谢物，不包括通过使用基于顶空固相微萃取的传统方法。(C)2015 Elsevier B. V.版权所有。
We developed a new device for the in vitro extraction of small molecule metabolites excreted from cancer cells. The extraction device, which was biocompatible and incubated with cancer cells, consists of a thin Tenax TA film deposited on the surface of a cylindrical aluminum rod. The Tenax TA solid phase was utilized for the direct extraction and preconcentration of the small molecule metabolites from a cell culture sample. The device fabrication and the metabolite extraction were optimized, tested, and validated using HeLa cell cultures. Comparison of metabolic profiles with the control measurement from the culture medium enabled detection of metabolites that were consumed or produced by the cell culture. Tentative identification and semi-quantitative investigation of the excreted metabolites were performed by GC-MS analysis. The proposed approach can be a valuable tool for the characterization of low-volatile cancer cell metabolites that are not covered by use of conventional methods based on headspace solid phase microextraction. (C) 2015 Elsevier B.V. All rights reserved.