Role of transglycosylation products in the expression of multiple xylanases in Myceliophthora sp IMI 387099
Role of transglycosylation products in the expression of multiple xylanases in Myceliophthora sp IMI 387099
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DOI：
10.1007/s00284-006-0204-5
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发表时间：
2007-06-01
影响因子：
2.6
通讯作者：
Bhat, M. K.
中科院分区：
文献类型：
--
作者：
Badhan, A. K.;Chadha, B. S.;Bhat, M. K.
本研究报道了由毁丝霉属物种IMI 387099产生的多种木聚糖酶的调节。以果糖为唯一碳源时，对复合木聚糖酶的表达有正向调节作用。酸性pI的木聚糖酶（EX 1和EX 2）在单糖（葡萄糖、阿拉伯糖和木糖）存在下表达，而酸性和碱性pI的木聚糖酶（EX 1、EX 2、EX 3和EX 5）在果糖、木聚糖以及木聚糖和醇的组合存在下表达。果糖和木聚糖的组合还导致另外的木聚糖酶（EX 4）的表达。位置异构体（iso-X4）被发现是关键的转糖基化产物时，培养物生长在果糖和木聚糖的存在下。在醇存在下，木聚糖酶的较高表达归因于存在于培养物提取物中的烷基糖苷和其他转糖基化产物的协同效应。
This study reports the regulation of multiple xylanases produced by Myceliophthora sp. IMI 387099. Fructose was found to positively regulate the expression of multiple xylanase when used as sole carbon source. The xylanases (EX1 and EX2) of acidic pI were expressed in the presence of simple sugars (glucose, arabinose, and xylose), whereas xylanase of both acidic as well as basic pI (EX1, EX2, EX3, and EX5) were expressed in the presence of fructose, xylan, and combination of xylan and alcohol. The combination of fructose and xylan also led to expression of an additional xylanase (EX4). The positional isomer (iso-X4) was found to be the key transglycosylation product when cultures were grown in the presence of fructose and xylan. In the presence of alcohols, the higher expression of xylanase was ascribed to the synergistic effect of alkyl glycoside and other transglycosylation products present in the culture extracts.