Levitation and movement of human tumor cells using a printed circuit board device based on software-controlled dielectrophoresis
Levitation and movement of human tumor cells using a printed circuit board device based on software-controlled dielectrophoresis
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DOI：
10.1002/bit.10590
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发表时间：
2003-05-20
影响因子：
3.8
通讯作者：
Gambari, R
中科院分区：
文献类型：
--
作者：
Altomare, L;Borgatti, M;Gambari, R
在这项研究中，我们描述了一种原创、高效、创新的印刷电路板 (PCB) 设备，能够生成基于介电泳、软件控制的笼子，可以将其移动到微室内的任何位置。根据真核细胞的介电泳特性，真核细胞可以被“捕获”在笼子中并在软件控制下移动。从报告的实验数据中得出的主要结论是，基于介电泳的PCB装置允许不同的肿瘤细胞在不同的介电泳条件下悬浮和运动。因此，本文提出的结果是旨在使用“芯片实验室”强制相互作用或分离真核细胞的实验的基础。事实上，因为可以同时控制许多笼子，并且可以迫使两个或多个笼子共享相同或不同的位置，所以原则上可以引入不同组织型的接触细胞或将它们分开。 (C) 2003 年 Wiley 期刊公司。
In this study we describe an original, efficient, and innovative printed circuit board (PCB) device able to generate dielectrophoresis-based, software-controlled cages that can be moved to any place inside a microchamber. Depending on their dielectrophoretic properties, eukaryotic cells can be "entrapped" in cages and moved under software control. The main conclusion gathered from the experimental data reported is that the PCB device based on dielectrophoresis permits levitation and movement of different tumor cells at different dielectrophoresis conditions. The results presented herein are therefore the basis for experiments aimed at forced interactions or separation of eukaryotic cells using "labon-a-chip." In fact, because many cages can be controlled at the same time, and two or more cages can be forced to share the same or a different location, it is possible, in principle, either to bring in contact cells of a differing histotype or to separate them. (C) 2003 Wiley Periodicals, Inc.