Determinants of cytoplasmic microtubule depolymerization during ciliogenesis in Chlamydomonas reinhardtii.
Determinants of cytoplasmic microtubule depolymerization during ciliogenesis in Chlamydomonas reinhardtii.
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莱茵衣藻纤毛发生过程中细胞质微管解聚的决定因素。
DOI：
10.1101/2023.04.07.536038
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Avasthi,Prachee
中科院分区：
文献类型：
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作者：
Dougherty,LarissaL;Avasthi,Prachee
纤毛的核心是微管，微管对于确定长度和协助纤毛组装和拆卸很重要;然而，微管对纤毛发生的另一个调节作用位于纤毛之外。微管细胞骨架是一种高度动态的结构，其快速聚合和解聚以协助细胞过程。这些过程已经在各种生物体中进行了研究，包括化学和遗传扰动。然而，这些都产生了相互矛盾的数据方面的作用，细胞质微管（CytoMT）和自由微管蛋白的动力学在纤毛发生。在这里，我们看看在纤毛发生和细胞质微管动力学之间的关系，在衣原体莱茵衣藻使用化学和机械扰动。我们发现，不仅可以稳定的CytoMT允许正常的纤毛组装，但高钙浓度和低pH值诱导的脱附导致CytoMT脱附分别从纤毛脱落。此外，我们发现，纤毛脱落通过机械剪切，纤毛再生早，尽管完整的CytoMT。我们的数据表明，细胞质微管是不是一个水槽的限制池的细胞质微管蛋白在衣原体，解聚发生后decilization是一个后果，而不是纤毛发生的要求，和完整的CytoMT在细胞质和近端纤毛支持更有效的纤毛组装。
At the core of cilia are microtubules which are important for establishing length and assisting ciliary assembly and disassembly; however, another role for microtubule regulation on ciliogenesis lies outside of the cilium. The microtubule cytoskeleton is a highly dynamic structure which polymerizes and depolymerizes rapidly to assist in cellular processes. These processes have been studied across various organisms with chemical as well as genetic perturbations. However, these have generated conflicting data in terms of the role of cytoplasmic microtubules (CytoMTs) and free tubulin dynamics during ciliogenesis. Here we look at the relationship between ciliogenesis and cytoplasmic microtubule dynamics in Chlamydomonas reinhardtii using chemical and mechanical perturbations. We find that not only can stabilized CytoMTs allow for normal ciliary assembly, but high calcium concentrations and low pH-induced deciliation cause CytoMTs to depolymerize separately from ciliary shedding. In addition, we find that ciliary shedding through mechanical shearing, cilia regenerate earlier despite intact CytoMTs. Our data suggests that cytoplasmic microtubules are not a sink for a limiting pool of cytoplasmic tubulin in Chlamydomonas, depolymerization that occurs following deciliation is a consequence rather than a requirement for ciliogenesis, and intact CytoMTs in the cytoplasm and the proximal cilium support more efficient ciliary assembly.