Experimental second passage of chronic wasting disease (CWDmule deer) agent to cattle
Experimental second passage of chronic wasting disease (CWDmule deer) agent to cattle
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DOI：
10.1016/j.jcpa.2005.07.001
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发表时间：
2006-01-01
影响因子：
0.8
通讯作者：
Richt, JA
中科院分区：
文献类型：
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作者：
Hamir, AN;Kunkle, RA;Richt, JA
为了比较牛第一代和第二代慢性消耗性疾病（CWDmule（鹿））的临床病理学结果，在早期实验中，用来自第一代CWD感染小牛的脑组织对6头小牛进行脑内接种。两只未接种小牛作为对照。接种动物在10-12个月后开始失去食欲和体重，随后有5只出现中枢神经系统（CNS）异常的临床体征。到16.5个月时，由于预后不良，所有牛均被安乐死。没有动物表现出海绵状脑病（SE）的显微镜下病变，但PrPres的检测在其中枢神经系统组织的免疫组织化学（IHC）和快速蛋白质印迹（WB）技术。因此，脑内接种牛不仅扩增CWS PrPres从黑尾鹿，但也开发了临床中枢神经系统的迹象，在没有SE病变。这种情况也发生在接种羊瘙痒病病原体的牛中。该研究证实，目前美国用于诊断牛海绵状脑病（BSE）的诊断技术可以检测到牛的CWD，如果它是自然发生的。此外，它提出了区分牛CWD与BSE的可能性，由于没有神经病理学病变和独特的多灶性分布的PrPres，如IHC所示，在这项研究中，似乎比WB技术更敏感。爱思唯尔有限公司出版
To compare clinicopathological findings in first and second passage chronic wasting disease (CWDmule (deer)) in cattle, six calves were inoculated intracerebrally with brain tissue derived from a first-passage CWD-affected calf in an earlier experiment. Two uninoculated calves served as controls. The inoculated animals began to lose both appetite and weight 10-12 months later, and five subsequently developed clinical signs of central nervous System (CNS) abnormality. By 16.5 months, all cattle had been subjected to euthanasia because of poor prognosis. None of the animals showed microscopical lesions of spongiform encephalopathy (SE) but PrPres was detected in their CNS tissues by immunohistochemistry (IHC) and rapid Western blot (WB) techniques. Thus, intracerebrally inoculated cattle not only amplified CWS PrPres from mule deer but also developed clinical CNS signs in the absence of SE lesions. This situation has also been shown to occur in cattle inoculated with the scrapie agent. The study confirmed that the diagnostic techniques currently used for diagnosis of bovine spongiform encephalopathy (BSE) in the US would detect CWD in cattle, should it occur naturally. Furthermore, it raised the possibility of distinguishing CWD from BSE in cattle, due to the absence of neuropathological lesions and to a distinctive multifocal distribution of PrPres, as demonstrated by IHC which, in this study, appeared to be more sensitive than the WB technique. Published by Elsevier Ltd.