THE ANTIBODY-RESPONSE OF NORMAL MICE TO IMMUNIZATION WITH SINGLE-STRANDED-DNA OF VARIOUS SPECIES ORIGIN
THE ANTIBODY-RESPONSE OF NORMAL MICE TO IMMUNIZATION WITH SINGLE-STRANDED-DNA OF VARIOUS SPECIES ORIGIN
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DOI：
10.1016/0090-1229(89)90034-2
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发表时间：
1989-06-01
期刊：
影响因子：
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通讯作者：
PISETSKY, DS
中科院分区：
文献类型：
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作者：
GILKESON, GS;GRUDIER, JP;PISETSKY, DS
为了进一步评估诱导抗DNA抗体的机制，测定了BALB/c小鼠对各种物种来源的单链DNA免疫的应答。用大肠杆菌DNA作为与佐剂中的甲基化BSA的复合物免疫的小鼠的抗DNA水平通过ELISA显着大于用小牛胸腺DNA类似免疫的小鼠的抗DNA水平。比较了小牛胸腺DNA、鸡血液DNA、产气荚膜梭菌DNA、大肠杆菌DNA、大肠杆菌DNA和大肠杆菌DNA复合物免疫小鼠的免疫应答。大肠杆菌、人胎盘或鲑鱼睾丸中的抗体水平表明，细菌DNA诱导的抗体水平最高。用ELISA法检测了E.抑制ELISA显示，大肠杆菌DNA有两个群体，一个结合大肠杆菌中的独特决定簇;另一种与所有检测的DNA上表达的决定簇交叉反应。这些结果表明，DNA分子，当与蛋白质载体复合时，其免疫原性潜力不同，可能是因为存在很少由哺乳动物宿主DNA呈现的独特序列或结构。
To further assess the mechanism for the induction of anti-DNA antibodies, the response of BALB/c mice to immunization with single-stranded DNA of various species origin was determined. Anti-DNA levels of mice immunized with Escherichia coli DNA as complexes with methylated BSA in adjuvant were significantly greater by ELISA than those from mice immunized similarly with calf thymus DNA. Furthermore, comparison of the responses of mice immunized with complexes of DNA from calf thymus, chicken blood, Clostridium perfringens, E. coli, human placenta, or salmon testes indicated that the bacterial DNAs induced the highest antibody levels. The antibody response to E. coli DNA was shown by inhibition ELISA to have two populations, one binding unique determinants in E. coli DNA and the other cross-reactive with determinants expressed on all DNAs tested. These results indicate that DNA molecules, when complexed to a protein carrier, differ in their immunogenic potential, likely because of the presence of unique sequences or structures rarely presented by mammalian host DNA.