Arabidopsis noncoding RNA mediates control of photomorphogenesis by red light
Arabidopsis noncoding RNA mediates control of photomorphogenesis by red light
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拟南芥非编码RNA介导红光对光形态发生的控制
DOI：
10.1073/pnas.1409457111
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发表时间：
2014-06
期刊：
影响因子：
--
通讯作者：
Deng, Xing Wang
中科院分区：
文献类型：
--
作者：
He, Guangming;Terzaghi, William;Zhu, Danmeng;Deng, Xing Wang
意义基因表达程序的动态调控在高等植物光介导的幼苗光形态建成过程中既是关键的，也是精确调控的。我们的工作将隐藏的宝藏1(HID1)，一种作为光形态建成的正调控因子的非编码RNA，添加到目前已知的控制光形态建成的关键遗传因素组中。具体地说，我们通过多种方法获得的数据表明，HID1通过直接抑制光敏色素相互作用因子3来调制红光介导的光形态建成，光敏色素相互作用因子3编码了一个关键的转录因子，抑制了光反应。HID1在高等植物中似乎高度保守。幼苗光形态建成是一个复杂的发育过程，由基因表达的转录和转录后调控共同控制。在这里，我们发现了一个拟南芥的非编码RNA，命名为隐藏的宝藏1(HID1)，作为一个促进连续红光(Cr)光形态建成的因子。我们发现HID1通过光敏色素相互作用因子3(PIF3)发挥作用，PIF3编码一个基本的螺旋-环-螺旋转录因子，已知是光形态建成的关键抑制因子。HID1突变体中HID1的敲除导致PIF3的表达显著增加，这反过来又驱动了cr伸长的下胚轴的发育。我们在HID1中确定了两个主要的茎环，这两个茎环对于它调节cr生长的幼苗的下胚轴生长是必不可少的。此外，我们的数据显示，HID1被组装成大的核蛋白-RNA复合体(ES)，并与PIF3第一内含子的染色质结合以抑制其转录。系统发育分析表明，许多陆地植物具有保守的HID1同源基因，其水稻同源基因在拟南芥HID1突变体中表达时可以挽救突变表型。因此，我们得出结论：HID1是一个以前未被描述的非编码RNA，它的功能代表了精确控制幼苗光形态建成的另一层调控。
Significance The dynamic regulation of gene-expression programs is both critical to and regulated precisely in the light-mediated seedling photomorphogenesis of higher plants. Our work adds HIDDEN TREASURE 1 (HID1), a noncoding RNA that acts as a positive regulator of photomorphogenesis, to the current group of pivotal genetic factors known to control photomorphogenesis. Specifically, our data obtained by numerous approaches reveal that HID1 modulates red light-mediated photomorphogenesis by directly repressing PHYTOCHROME-INTERACTING FACTOR 3, which encodes a key transcription factor that inhibits red light responses. HID1 appears to be highly conserved among higher plants. Seedling photomorphogenesis is a sophisticated developmental process that is controlled by both the transcriptional and posttranscriptional regulation of gene expression. Here, we identify an Arabidopsis noncoding RNA, designated HIDDEN TREASURE 1 (HID1), as a factor promoting photomorphogenesis in continuous red light (cR). We show that HID1 acts through PHYTOCHROME-INTERACTING FACTOR 3 (PIF3), which encodes a basic helix–loop–helix transcription factor known to be a key repressor of photomorphogenesis. Knockdown of HID1 in hid1 mutants leads to a significant increase in the expression of PIF3, which in turn drives the development of elongated hypocotyls in cR. We identified two major stem-loops in HID1 that are essential for its modulation of hypocotyl growth in cR-grown seedlings. Furthermore, our data reveal that HID1 is assembled into large nuclear protein–RNA complex(es) and that it associates with the chromatin of the first intron of PIF3 to repress its transcription. Strikingly, phylogenetic analysis reveals that many land plants have conserved homologs of HID1 and that its rice homolog can rescue the mutant phenotype when expressed in Arabidopsis hid1 mutants. We thus concluded that HID1 is a previously uncharacterized noncoding RNA whose function represents another layer of regulation in the precise control of seedling photomorphogenesis.
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影响因子：
64.8
作者：
通讯作者：
--
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
64.5
作者：
Ji X;Zhou Y;Pandit S;Huang J;Li H;Lin CY;Xiao R;Burge CB;Fu XD
通讯作者：
Fu XD
拟南芥中中等大小非编码RNA的基因组特征和调控作用
影响因子：
27.5
作者：
Wang, Yuqiu;Wang, Xuncheng;Deng, Xing Wang
通讯作者：
Deng, Xing Wang
DOI：
10.1073/pnas.0812219106
发表时间：
2009-05-05
影响因子：
11.1
作者：
Shin, Jieun;Kim, Keunhwa;Choi, Giltsu
通讯作者：
Choi, Giltsu