Comprehensive functional analysis of the catalase gene family in Arabidopsis thaliana
Comprehensive functional analysis of the catalase gene family in Arabidopsis thaliana
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拟南芥过氧化氢酶基因家族的综合功能分析
DOI：
10.1111/j.1744-7909.2008.00741.x
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发表时间：
2008-10-01
影响因子：
11.4
通讯作者：
Song, Chun-Peng
中科院分区：
文献类型：
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作者：
Du, Yan-Yan;Wang, Peng-Cheng;Song, Chun-Peng
在拟南芥中，过氧化氢酶（catalase，CAT）基因编码一个小家族的蛋白质，包括CAT 1、CAT 2和CAT 3，它们催化过氧化氢（hydrogen peroxide，H2O2）的分解并在控制活性氧（reactive oxygen species，ROS）的稳态中发挥重要作用。本文分析了干旱、低温、氧化胁迫、脱落酸和水杨酸等处理对拟南芥过氧化氢酶的影响。我们的研究结果表明，CAT1是一个重要的球员在各种环境压力下产生的过氧化氢的去除。CAT2和CAT3是主要的H2O2清除剂，分别在光或黑暗中促进ROS稳态。此外，CAT 2被低温和干旱胁迫激活，CAT 3主要被脱落酸和氧化处理以及衰老阶段增强。这些结果，加上以前的数据，表明转录控制网络解释了如何CAT和其他清道夫酶，如过氧化物酶和超氧化物歧化酶可能协调调节在发展过程中，但差异表达不同的压力控制ROS稳态。
In Arabidopsis, catalase (CAT) genes encode a small family of proteins including CAT1, CAT2 and CAT3, which catalyze the decomposition of hydrogen peroxide (H2O2) and play an important role in controlling homeostasis of reactive oxygen species (ROS). Here, we analyze the expression profiles and activities of three catalases under different treatments including drought, cold, oxidative stresses, abscisic acid and salicylic acid in Arabidopsis. Our results reveal that CAT1 is an important player in the removal of H2O2 generated under various environmental stresses. CAT2 and CAT3 are major H2O2 scavengers that contribute to ROS homeostasis in light or darkness, respectively. In addition, CAT2 is activated by cold and drought stresses and CAT3 is mainly enhanced by abscisic acid and oxidative treatments as well as at the senescence stage. These results, together with previous data, suggest that the network of transcriptional control explains how CATs and other scavenger enzymes such as peroxidase and superoxide dismutase may be coordinately regulated during development, but differentially expressed in response to different stresses for controlling ROS homeostasis.