Simultaneous identification of Fritillariae cirrhosae bulbus and its common adulterants in one reaction by multiplex ligation-dependent probe amplification and high-resolution melting curve assay.
Simultaneous identification of Fritillariae cirrhosae bulbus and its common adulterants in one reaction by multiplex ligation-dependent probe amplification and high-resolution melting curve assay.
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DOI：
10.1016/j.gene.2021.145620
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发表时间：
2021-03
期刊：
影响因子：
3.5
通讯作者：
Hailan Zhu;Wenbin Wang;Yuxin Zhou;Bo Wang;J. Nie
中科院分区：
文献类型：
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作者：
Hailan Zhu;Wenbin Wang;Yuxin Zhou;Bo Wang;J. Nie
贝母是我国著名的名贵药食两用药材，具有显著的消肿止咳作用，且副作用较同类药少。由于其价格昂贵和产量有限，它一直受到各种廉价同类掺假的影响。本文介绍了一种快速、高通量、灵敏、高效的同时鉴定F.采用多重连接依赖探针扩增结合高分辨熔解曲线（MLPA-HRM）技术，对何首乌及其常见混淆品的ITS 1区进行了分析。该方法灵敏度高，检出限为0.19 ng，特异性强，与常见的掺假品无交叉反应。混合样品分析表明，F.一种MLPA-HRM反应。总之，本文所述的方法非常适合于检测掺假在F. tissaebulbus。
Fritillariae cirrhosae bulbus, a well-known and precious medicinal and edible herb in China, causes remarkable effects on swelling and relieving cough, with fewer side effects than other congeneric medicine. It has been subject to various cheaper congeneric adulteration because of its high price and limited production. In this paper, a rapid, high throughput, sensitive and efficient technique was described for simultaneous identification ofF. cirrhosae bulbusand its common adulterants by employing multiplex ligation-dependent probe amplification coupled with high-resolution melting (MLPA-HRM) curve assay in their internal transcribed spacer 1 (ITS1) regions. This assay was highly sensitive with a detection limit of 0.19 ng genomic DNA, and highly specific with no cross-reaction with common adulterants. Mixed sample analysis showed as low as 10% adulteration can be detected fromF. cirrhosae bulbusin one MLPA-HRM reaction. Overall, the method described in this paper is well suited for detecting adulteration inF. cirrhosae bulbus.