Schistosome satellite DNA encodes active hammerhead ribozymes
Schistosome satellite DNA encodes active hammerhead ribozymes
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DOI：
10.1128/mcb.18.7.3880
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发表时间：
1998-07-01
影响因子：
5.3
通讯作者：
Cedergren, R
中科院分区：
文献类型：
--
作者：
Ferbeyre, G;Smith, JM;Cedergren, R
利用设计用于在序列数据库中搜索RNA结构基序的计算机程序，我们在曼氏血吸虫Smα重复DNA中发现了一个锤头状核酶结构域。这些重复序列的转录本被表达为在体外和体内裂解成单位长度片段的长多聚体前体RNA。这个RNA结构域能够参与锤头状核酶典型的顺式和反式切割。对曼氏血吸虫DNA的进一步计算机分析确定了编码突触素样蛋白的基因中有一个潜在的反式切割位点，从该基因转录的RNA被Sma！核酶在体外。在血吸虫和杜氏血吸虫中发现了相似的包含锤头结构域的重复序列家族，但在日本血吸虫和美国异种血吸虫中没有发现，这表明锤头结构域不是从共同的血吸虫祖先那里获得的。
Using a computer program designed to search for RNA structural motifs in sequence databases, we have found a hammerhead ribozyme domain encoded in the Sm alpha repetitive DNA of Schistosoma mansoni. Transcripts of these repeats are expressed as long multimeric precursor RNAs that cleave in vitro and in vivo into unit-length fragments. This RNA domain is able to engage in both cis and trans cleavage typical of the hammerhead ribozyme. Further computer analysis of S. mansoni DNA identified a potential trans cleavage site in the gene coding for a synaptobrevin-like protein, and RNA transcribed from this gene was efficiently cleaved by the Sma! ribozyme in vitro. Similar families of repeats containing the hammerhead domain were found in the closely related Schistosoma haematobium and Schistosomatium douthitti species but were not present in Schistosoma japonicum or Heterobilharzia americana, suggesting that the hammerhead domain was not acquired from a common schistosome ancestor.