Quantitative gene expression analysis by cDNA microarray during liver regeneration after partial hepatectomy in rats
Quantitative gene expression analysis by cDNA microarray during liver regeneration after partial hepatectomy in rats
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DOI：
10.1007/s00595-004-2962-7
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发表时间：
2005-05-01
期刊：
影响因子：
2.5
通讯作者：
Chen, WJ
中科院分区：
文献类型：
--
作者：
Lai, HS;Chen, Y;Chen, WJ
目的。据推测，基因调控在肝再生过程中发挥着重要作用。我们进行这项研究是为了分析部分肝切除术后肝再生过程中的定量基因表达。方法。六十只雄性 Wistar 大鼠被随机分为十组，每组六只。一组大鼠在术前处死，另外九组分别在70%部分肝切除术后2、4、6、12、24、48和72小时以及5和7天处死。分离剩余肝脏用于mRNA提取。通过阵列机制备携带6144个聚合酶链式反应扩增的cDNA片段的cDNA微阵列来进行基因表达的质量调查。使用平板扫描仪对微阵列图像进行扫描、数字化和分析。结果。基因表达的变化被分为 72 种不同的模式，包括单峰模式、部分肝切除后 2、4、6、12 和 72 小时以及 5 和 7 天。其他形态包括双峰、增强趋势、减弱趋势、凸曲线、挖掘曲线以及各种混合类型。每类基因表达模式包含40-218个不同的原癌基因。结论。定量基因表达谱具有重要意义，需要进一步研究肝再生过程中涉及的遗传机制。
Purpose. It is speculated that genetic regulation plays an important role during liver regeneration. We conducted this study to analyze quantitative gene expression during liver regeneration after partial hepatectomy.Methods. Sixty male Wistar rats were randomly assigned into ten groups of six. One group of rats was killed preoperatively and the other nine groups were killed 2, 4, 6, 12, 24, 48, and 72 h, and 5 and 7 days after 70% partial hepatectomy, respectively. The remnant livers were isolated for mRNA extraction. A mass survey of gene expression by cDNA microarray carrying 6144 polymerase chain reaction-amplified cDNA fragments was prepared by an arraying machine. The microarray images were scanned, digitized, and analyzed using a flatbed scanner.Results. The variations in gene expression were classified into 72 different patterns including a pattern with a single peak, 2, 4, 6, 12, and 72 h, and 5 and 7 days after partial hepatectomy. The other patterns included double peaks, enhancing trend, diminished trend, protruding curve, excavated curve, and various mixed types. Each category of gene expression pattern contained 40 - 218 different proto-oncogenes.Conclusion. The quantitative gene expression profiles have important implications, warranting further investigation of the genetic mechanisms involved in the process of liver regeneration.